Analytical Data
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Gene name
LSM3
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简介
The LSM3 protein plays a crucial role in pre-mRNA splicing as a component of the U4/U6-U5 tri-snRNP complex involved in spliceosome assembly and as part of the precatalytic spliceosome (spliceosome B complex). The heptameric LSM2-8 complex (including LSM3) specifically binds to the 3'-end U strand of U6 snRNA. LSM3 Protein, Human (His) is the recombinant human-derived LSM3 protein, expressed by E. coli , with N-His labeled tag.
- Application
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Alternative Names
U6 snRNA-associated Sm-like protein LSm3; LSM3; MDS017
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Species
Human
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Source
E. coli
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Tag
N-His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P62310
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Expression Region
M1-G102
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Protein Length
Full Length
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Molecular Weight
16 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The LSM3 protein, part of the LSm (Like-Sm) family, plays a crucial role in RNA metabolism, including mRNA processing, splicing, and degradation. It is a key component of the cytoplasmic LSm complex, which is involved in the regulation of RNA stability and translation, impacting gene expression. LSM3 is evolutionarily conserved across various species, indicating its fundamental importance in cellular functions. Recent studies have highlighted its involvement in stress responses and cellular signaling pathways, suggesting a broader functional repertoire beyond RNA processing. Researchers are increasingly interested in the recombinant expression of LSM3 to elucidate its structure-function relationships, as well as its interactions with RNA and other regulatory proteins. Understanding the functional dynamics of LSM3 can provide insights into the mechanisms underlying gene regulation and the pathogenesis of various diseases, including cancer and genetic disorders. This research bears significance not only for basic molecular biology but also for potential therapeutic applications, especially in targeting RNA metabolism in disease contexts. As the field of RNA biology expands, the detailed investigation of LSM3 and its recombinant forms is expected to advance our understanding of post-transcriptional regulation and its implications in health and disease.
