Analytical Data
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Gene name
SETD7
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简介
The SETD7 protein is a histone methyltransferase that specifically monomethylates H3K4, a mark associated with transcriptional activation. Its role in gene activation extends to the collagenase and insulin genes, specifically at the insulin promoter when IPF1/PDX-1 is recruited. SETD7 Protein, Human (His) is the recombinant human-derived SETD7 protein, expressed by E. coli , with N-His labeled tag.
- Application
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Alternative Names
Histone-lysine N-methyltransferase SETD7; H3-K4-HMTase SETD7; SET7/9; KIAA1717; KMT7; SET7; SET9
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Species
Human
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Source
E. coli
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Tag
N-His
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Purity
Greater than 95% as determined by SDS-PAGE.
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Uniprot
Q8WTS6
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Expression Region
D2-K366
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AA Sequence
DSDDEMVEEAVEGHLDDDGLPHGFCTVTYSSTDRFEGNFVHGEKNGRGKFFFFDGSTLEGYYVDDALQGQGVYTYEDGGVLQGTYVDGELNGPAQEYDTDGRLIFKGQYKDNIRHGVCWIYYPDGGSLVGEVNEDGEMTGEKIAYVYPDERTALYGKFIDGEMIEGKLATLMSTEEGRPHFELMPGNSVYHFDKSTSSCISTNALLPDPYESERVYVAESLISSAGEGLFSKVAVGPNTVMSFYNGVRITHQEVDSRDWALNGNTLSLDEETVIDVPEPYNHVSKYCASLGHKANHSFTPNCIYDMFVHPRFGPIKCIRTLRAVEADEELTVAYGYDHSPPGKSGPEAPEWYQVELKAFQATQQK
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Protein Length
Partial
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Molecular Weight
47-50 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
SETD7, also known as SET domain containing 7, is a lysine methyltransferase that plays a crucial role in regulating gene expression through histone modification. Its primary function is to methylate lysine 4 on histone H3 (H3K4), a mark associated with active transcription. SETD7 is implicated in various biological processes, including development, cell differentiation, and response to stress, highlighting its importance in maintaining cellular homeostasis. Dysregulation of SETD7 has been linked to several diseases, including cancer, neurodegenerative disorders, and metabolic diseases, making it a potential therapeutic target. Recent studies have focused on the structural and functional characterization of SETD7, revealing insights into its enzymatic mechanism and interaction with substrates. The recombinant expression of SETD7 protein allows researchers to produce large quantities for functional assays and to investigate its role in cellular contexts. Understanding SETD7's involvement in histone methylation not only enhances our knowledge of epigenetic regulation but also opens avenues for the development of small molecule inhibitors that could modulate its activity in disease settings. This protein's study represents a significant step in comprehending the complex regulatory networks governing gene expression and the potential for targeted epigenetic therapies.
