Analytical Data
-
基因名
speA
-
简介
The speA protein plays a crucial role in catalyzing the transformation from arginine to agmatine. This enzymatic conversion highlights speA's significance in sustaining cellular metabolism and essential biological processes. By synthesizing agmatine, speA emerges as a key player in the intricate dance of molecular transformations that characterize fundamental cellular functions. speA Protein, Shewanella putrefaciens (FLAG, His) is the recombinant speA protein, expressed by E. coli , with N-6*His, N-Flag labeled tag.
- Application
-
别名
speA; Biosynthetic arginine decarboxylase; ADC
-
种属
Others
-
表达系统
E. coli
-
标签
N-6*His;N-Flag
-
纯度
Greater than 90% as determined by SDS-PAGE.
-
蛋白编号
A4Y5Y9
-
表达区间
M1-S637
-
蛋白长度
Full Length
-
内毒素
< 1.0 EU per μg protein as determined by the LAL method.
-
性状
Freeze-dried powder
-
缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
-
稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
The study of the recombinant protein speA (Streptococcus pyogenes exotoxin A) has gained significant attention due to its role as a virulence factor in the pathogenesis of Streptococcal infections. Streptococcus pyogenes, a bacterium responsible for various infections ranging from mild pharyngitis to severe conditions like necrotizing fasciitis and toxic shock syndrome, produces speA, which can function as a superantigen. This property allows speA to activate a large number of T cells, leading to an overwhelming immune response that can result in severe inflammatory reactions. The recombinant production of speA enables researchers to study its structure, function, and interaction with immune cells in vitro and in vivo, facilitating a deeper understanding of its mechanisms of action and potential therapeutic applications. Additionally, investigating the biochemical properties of speA can aid in the development of vaccine strategies and novel treatments against Streptococcus pyogenes infections. The insights gained from these studies can contribute to improved clinical outcomes and public health interventions targeting this pathogenic bacterium.
