Analytical Data
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基因名
FGFR-1 alpha (IIIb)
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简介
FGFR-1 alpha, a conserved member of the FGFR family, binds acidic and basic fibroblast growth factors, influencing mitogenesis and differentiation. Mutations in FGFR1 cause syndromes and disorders. It exhibits ubiquitous expression, with notable levels in ovary (RPKM 21.8), fat (RPKM 21.4), and 25 other tissues. Alternatively spliced variants contribute to its functional diversity. FGFR-1 alpha (IIIb) Protein, Human (HEK293, His) is a recombinant protein dimer complex containing human-derived FGFR-1 alpha, expressed by HEK293 , with C-His, C-10*His labeled tag. FGFR-1 alpha (IIIb) Protein, Human (HEK293, His), has molecular weight of 60-90 kDa.
- Application
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生物活性
Measured by its ability to inhibit FGF acidic-dependent proliferation of NIH/3T3 mouse fibroblast cells. The ED50 for this effect is 4.134 ng/mL, corresponding to a specific activity is 2.42×10^5 units/mg. Measured by its ability to inhibit FGF acidic-dependent proliferation of NIH/3T3 mouse fibroblast cells. The ED50 for this effect is 4.134 ng/mL, corresponding to a specific activity is 2.42×105 units/mg.
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别名
Fibroblast growth factor receptor 2; FGFR-2; FGF R2a; FGFR2 alpha
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种属
Human
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表达系统
HEK293
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标签
C-His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P11362-7
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表达区间
P11362-7/NP_056934.2 (R22-K310&A359-E374)&AAB19502 (H1-P47)
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氨基酸序列
A1: RPSPTLPEQAQPWGAPVEVESFLVHPGDLLQLRCRLRDDVQSINWLRDGVQLAESNRTRITGEEVEVQDSVPADSGLYACVTSSPSGSDTTYFSVNVSDALPSSEDDDDDDDSSSEEKETDNTKPNPVAPYWTSPEKMEKKLHAVPAAKTVKFKCPSSGTPNPTLRWLKNGKEFKPDHRIGGYKVRYATWSIIMDSVVPSDKGNYTCIVENEYGSINHTYQLDVVERSPHRPILQAGLPANKTVALGSNVEFMCKVYSDPQPHIQWLKHIEVNGSKIGPDNLPYVQILK A2: ALEERPAVMTSPLYLE A3: HSGINSSDAEVLTLFNVTEAQSGEYVCKVSNYIGEANQSAWLTVTRP
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分子量
60-90 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Fibroblast growth factor receptor 1 (FGFR-1) is a crucial member of the fibroblast growth factor receptor family that plays pivotal roles in various biological processes, including cell proliferation, differentiation, and survival. The FGFR-1 alpha isoform, specifically the IIIb variant, is predominantly expressed in epithelial tissues and is involved in developmental processes as well as the maintenance of tissue homeostasis. Dysregulation or mutations in FGFR-1 have been implicated in several pathological conditions, including various forms of cancer, where aberrant signaling contributes to tumor growth and metastasis. Research into the recombinant expression of FGFR-1 alpha (IIIb) has gained attention as it provides insights into the receptor’s structure-function relationships and its interactions with specific ligands, such as fibroblast growth factors (FGFs). Producing this recombinant protein allows for detailed biochemical studies and the exploration of its signaling pathways, which may reveal potential therapeutic targets or biomarker candidates for FGFR-related diseases. Recent advances in protein engineering techniques have facilitated the generation of high-purity FGFR-1 alpha (IIIb), enabling further investigations into its role in cellular signaling and its potential implications in targeted therapies. As understanding of FGFR-1 alpha (IIIb) increases, it holds promise not only in the realm of cancer biology but also in regenerative medicine and tissue engineering, making it a significant focus of contemporary biomedical research.
