Analytical Data
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Gene name
DNASEII
- Application
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Alternative Names
DNase-II; DNase2; DNASE2A; DNASE2B; DRN2; DNL; DNL2; Deoxyribonuclease II Alpha; Acid DNase; Lysosomal DNase II
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Species
Human
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Source
E. coli
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Tag
N-His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O00115
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Expression Region
Cys19~Ile360
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Molecular Weight
42kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
DNase II is a crucial endonuclease that plays a significant role in DNA degradation and disposal within cellular processes, particularly in lysosomal degradation. Unlike other DNases, DNase II functions optimally in acidic environments, making it essential for the degradation of extracellular DNA, which can arise from cellular debris, apoptotic cells, and pathogen infection. The study of recombinant DNase II proteins has garnered attention due to their potential applications in various fields, including biotechnology, medicine, and genetic research. For instance, understanding the enzyme’s structure and function can lead to innovative therapeutic strategies in treating genetic disorders or enhancing the efficacy of gene therapy. Additionally, recombinant DNase II can serve as a tool for studying cellular processes linked to DNA metabolism, inflammation, and immune responses. Recent advances in molecular biology techniques enable the production and purification of high-quality DNase II recombinant proteins, allowing for detailed biochemical characterization and functional assays. Overall, the research surrounding DNase II recombinant proteins continues to expand, providing insights into their biological significance and potential biotechnological applications.
