Analytical Data
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基因名
rpmF
- Application
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别名
rpmF; b1089; JW1075; 50S ribosomal protein L32; Large ribosomal subunit protein bL32
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种属
Escherichia coli
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表达系统
E. coli
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标签
N- GST
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P0A7N4
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表达区间
2-57aa
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分子量
33.3 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The rpmF gene encodes a vital ribosomal protein, specifically the L32 protein in bacteria, which plays a significant role in the assembly and function of the ribosome, the cellular machinery responsible for protein synthesis. Research into the rpmF recombinant protein is pivotal due to its implications in understanding bacterial translation processes and antibiotic resistance mechanisms. Given the increasing prevalence of antibiotic-resistant bacteria, studying the structural and functional properties of ribosomal proteins, including rpmF, provides insights into potential targets for novel antimicrobial therapies. The recombinant production of the rpmF protein allows researchers to explore its interaction with other ribosomal components and factors involved in translation, further elucidating its role in ribosome stability and function. Additionally, the characterization of rpmF contributes to our broader understanding of ribosomal structure and function across various bacterial species, potentially leading to advancements in biotechnology and synthetic biology applications.
