Cat: IPD-X38868

Recombinant Human ORNT1 Protein,His

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Analytical Data

  • Gene name

    ORNT1

  • Application

    SPRMSTBLIITCELISACELL ASSAYDRUG SCREENING

  • Alternative Names

    SLC25A15; HHH; ORC1; Solute Carrier Family 25 Member 15

  • Species

    Human

  • Source

    E. coli

  • Tag

    N-His

  • Purity

    Greater than 90% as determined by SDS-PAGE.

  • Uniprot

    Q9Y619

  • Expression Region

    Met1~Tyr301

  • Molecular Weight

    33kDa

  • Endotoxin

    < 1.0 EU per μg protein as determined by the LAL method.

  • Form

    Freeze-dried powder

  • Buffer formulation

    PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

  • Reconstitution

    Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

  • Customization

    Site-directed mutagenesis Custom tag design Custom buffer formulation Custom full-length protein production

  • Stability Test

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.

  • Storage & Shelf Life

    Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

  • Shipping

    In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.

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Protein Description

ORNT1 (octanoyltransferase 1) is an enzyme that plays a significant role in mitochondrial fatty acid metabolism and is essential for the biosynthesis of mitochondrial translation factors. Its primary function involves the transfer of acyl groups, particularly octanoyl groups, to specific proteins, which is crucial for the post-translational modification of these proteins and their subsequent function in mitochondrial protein synthesis. Research into ORNT1 has gained momentum due to its implications in various metabolic disorders, mitochondrial dysfunctions, and their associated pathologies. Understanding the structure and function of ORNT1 is vital for elucidating its role in cellular energy metabolism and the regulation of fatty acid synthesis. Furthermore, altered expression or mutations in ORNT1 have been linked to several diseases, including diabetes and cardiomyopathy, highlighting its potential as a therapeutic target. The recombinant production of ORNT1 allows for in-depth studies that can provide insights into its enzymatic mechanisms and interactions with other mitochondrial proteins. This research also paves the way for the development of novel treatments for conditions linked to mitochondrial dysfunction. By utilizing recombinant DNA technology, scientists can produce large quantities of ORNT1, enabling biochemical assays and structural studies that may reveal critical information about its function and regulation in lipid metabolism and overall mitochondrial health. Ultimately, a comprehensive understanding of ORNT1's role could lead to advancements in the treatment of metabolic disorders and better strategies for managing diseases associated with mitochondrial dysfunction.

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