Analytical Data
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Gene name
PIAS4
- Application
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Alternative Names
PIASY; Piasg; ZMIZ6; Zinc Finger,MIZ-Type Containing 6; Protein inhibitor of activated STAT protein gamma; E3 SUMO-protein ligase PIAS4
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Species
Mouse
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Source
E. coli
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Tag
N-His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9JM05
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Expression Region
Val12~Leu272
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Molecular Weight
36kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
PIAS4 (Protein Inhibitor of Activated STAT4) is a member of the PIAS family of proteins, which are known to modulate various signaling pathways, particularly those involved in immune responses and cytokine signaling. Its primary function is to act as an E3 ubiquitin ligase that promotes the SUMOylation of specific target proteins, thereby regulating transcription and maintaining cellular homeostasis. Research has highlighted the role of PIAS4 in the modulation of signal transducer and activator of transcription (STAT) signaling pathways, particularly STAT4, which is critical for T-cell differentiation and the immune response against infections. Dysregulation of PIAS4 has been implicated in various diseases, including autoimmune disorders and cancers. Understanding the structure and function of PIAS4, as well as its interaction with other proteins and the molecular mechanisms underlying its activity, is essential for elucidating its biological roles. The development of recombinant PIAS4 proteins provides a valuable tool for studying its function and regulation in cellular systems, as well as for exploring potential therapeutic targets in immune-related diseases. Current research aims to clarify these aspects through biochemical analyses, structural studies, and in vivo models, contributing to a deeper understanding of PIAS4 and its potential implications in biomedicine.
