Analytical Data
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Gene name
USF1
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简介
The USF1 protein is a transcription factor that binds symmetric E-box DNA (5'-CACGTG-3') in viral and cellular promoters. Efficient DNA binding requires dimerization, typically using bHLH proteins. USF1 Protein, Human (His) is the recombinant human-derived USF1 protein, expressed by E. coli , with N-6*His labeled tag.
- Application
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Alternative Names
USF1; upstream transcription factor 1; UEF; FCHL; MLTF; FCHL1; MLTFI; HYPLIP1; bHLHb11; OTTHUMP00000029691; OTTHUMP00000029693; USF; Major late transcription factor 1; upstream stimulatory factor 1
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Species
Human
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Source
E. coli
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Tag
N-6*His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P22415
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Expression Region
G3-N310
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AA Sequence
GQQKTAETEEGTVQIQEGAVATGEDPTSVAIASIQSAATFPDPNVKYVFRTENGGQVMYRVIQVSEGQLDGQTEGTGAISGYPATQSMTQAVIQGAFTSDDAVDTEGTAAETHYTYFPSTAVGDGAGGTTSGSTAAVVTTQGSEALLGQATPPGTGQFFVMMSPQEVLQGGSQRSIAPRTHPYSPKSEAPRTTRDEKRRAQHNEVERRRRDKINNWIVQLSKIIPDCSMESTKSGQSKGGILSKACDYIQELRQSNHRLSEELQGLDQLQLDNDVLRQQVEDLKNKNLLLRAQLRHHGLEVVIKNDSN
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Protein Length
Partial
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
USF1 (Upstream Stimulating Factor 1) is a transcription factor that plays a critical role in the regulation of various metabolic processes, including glucose and lipid metabolism. Its significance has been underscored by its association with metabolic disorders such as obesity, diabetes, and cardiovascular diseases. Research has shown that USF1 influences the expression of genes involved in insulin signaling and fatty acid synthesis, thereby impacting energy homeostasis in the body. Given the growing prevalence of metabolic diseases worldwide, understanding the molecular mechanisms underlying USF1 function has become increasingly important. Studies have revealed that USF1 interacts with several key signaling pathways and transcriptional regulators, suggesting that it may serve as a potential therapeutic target for intervention in metabolic syndromes. Moreover, the investigation of USF1's role in various tissues, particularly in the liver and adipose tissue, has provided insights into its diverse physiological functions. As researchers continue to explore USF1's structural and functional properties, the potential for developing USF1-based strategies to combat metabolic diseases becomes more promising, highlighting the relevance of this protein in the field of biomedical research.
