Analytical Data
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Gene name
ERCC5
- Application
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Alternative Names
DNA excision repair protein ERCC-5 Xeroderma pigmentosum group G-complementing protein
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Species
Human
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Source
E. coli
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Tag
N- His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P28715
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Expression Region
947-1186aa
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Molecular Weight
30.8 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
ERCC5, also known as XPG, is a crucial gene involved in the nucleotide excision repair (NER) pathway, which is essential for repairing DNA damage caused by environmental factors such as UV radiation and chemical mutagens. Mutations in the ERCC5 gene can lead to several disorders, most notably xeroderma pigmentosum (XP), a condition characterized by extreme sensitivity to sunlight and a significantly increased risk of skin cancer. Understanding the function and regulation of ERCC5 is critical for elucidating the mechanisms of DNA repair and its role in maintaining genomic stability. Recently, research efforts have focused on the recombinant expression of the ERCC5 protein, allowing scientists to study its structure, function, and interactions with other proteins in detail. These studies employ various biophysical techniques to examine the protein's enzymatic activity and its role in DNA repair processes. Additionally, by generating recombinant ERCC5, researchers can explore potential therapeutic strategies for diseases linked to NER deficiencies. This research not only has implications for understanding the molecular basis of certain genetic disorders but also paves the way for developing targeted treatments that enhance DNA repair mechanisms, ultimately offering hope for patients affected by conditions related to impaired DNA repair pathways.
