Analytical Data
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基因名
polC
- Application
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别名
polC; CENSYa_1827DNA polymerase II large subunit; Pol II; EC 2.7.7.7; Exodeoxyribonuclease large subunit; EC 3.1.11.1
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种属
Cenarchaeum symbiosum
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表达系统
E. coli
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标签
Tag Free
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
A0RYM0
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表达区间
287-832aa
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分子量
59 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The study of polC recombinant proteins is rooted in the broader field of molecular biology and genetics, particularly in the context of DNA replication and repair. PolC, a DNA polymerase found in various organisms, such as bacteria, plays a crucial role in the synthesis of DNA during cell division. Understanding its structure and function is essential for elucidating mechanisms of genome replication and maintaining genetic stability. Researchers have focused on characterizing polC to explore its enzymatic properties, fidelity, and interaction with other proteins involved in the DNA replication machinery. The ability to express polC as a recombinant protein allows for detailed biochemical analysis and structural studies, enabling scientists to investigate how mutations and variations influence its function. Furthermore, insights derived from polC research have implications for biotechnology, synthetic biology, and medicine, including the development of novel antibiotics that target bacterial replication processes. As such, the exploration of polC recombinant proteins not only deepens our understanding of fundamental biological processes but also holds potential for applied innovations in disease treatment and genetic engineering.












