Analytical Data
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Gene name
ACADSB
- Application
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Alternative Names
ACADSB；Short/branched chain specific acyl-CoA dehydrogenase. mitochondrial
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P45954
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Expression Region
34-432aa
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AA Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMKSSQS EALLNITNNG IHFAPLQTFT DEEMMIKSSV KKFAQEQIAP LVSTMDENSK MEKSVIQGLF QQGLMGIEVD PEYGGTGASF LSTVLVIEEL AKVDASVAVF CEIQNTLINT LIRKHGTEEQ KATYLPQLTT EKVGSFCLSE AGAGSDSFAL KTRADKEGDY YVLNGSKMWI SSAEHAGLFL VMANVDPTIG YKGITSFLVD RDTPGLHIGK PENKLGLRAS STCPLTFENV KVPEANILGQ IGHGYKYAIG SLNEGRIGIA AQMLGLAQGC FDYTIPYIKE RIQFGKRLFD FQGLQHQVAH VATQLEAARL LTYNAARLLE AGKPFIKEAS MAKYYASEIA GQTTSKCIEW MGGVGYTKDY PVEKYFRDAK IGTIYEGASN IQLNTIAKHI DAEY
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Molecular Weight
46 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
ACADSB (Acyl-CoA dehydrogenase, short-chain, family member 2) is an enzyme that plays a crucial role in the metabolism of fatty acids, particularly in the oxidation of medium- and short-chain acyl-CoA substrates. Its importance in human health is underscored by its association with fatty acid oxidation disorders, which can lead to severe metabolic anomalies. Research has shown that mutations in the ACADSB gene can impair its function, resulting in conditions like hyperinsulinemic hypoglycemia and other metabolic syndromes. Furthermore, understanding the structure and function of ACADSB at the molecular level can provide valuable insights into its catalytic mechanism and regulatory processes. This knowledge not only aids in elucidating the biochemical pathways involved in lipid metabolism but also enhances the potential for developing therapeutic interventions for related diseases. As the interest in lipid metabolism and its impacts on metabolic health continues to grow, the study of ACADSB as a model for investigating enzyme activity and dysfunction becomes increasingly relevant. Ultimately, the ongoing research into the recombinant protein forms of ACADSB aims to characterize its biochemical properties and interactions, paving the way for potential clinical applications in diagnosing and treating metabolic disorders linked to fatty acid metabolism.
