Analytical Data
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基因名
AKT1
- Application
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别名
AKT1；PKB；RAC；RAC-alpha serine/threonine-Protein kinase
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种属
Mouse
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表达系统
E. coli
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标签
N-terminal His Tag
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P31750
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表达区间
1-480aa
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氨基酸序列
MNDVAIVKEGWLHKRGEYIKTWRPRYFLLKNDGTFIGYKERPQDVDQRESPLNN FSVAQCQLMKTERPRPNTFIIRCLQWTTVIERTFHVETPEEREEWATAIQTVAD GLKRQEEETMDFRSGSPSDNSGAEEMEVSLAKPKHRVTMNEFEYLKLLGKGTFG KVILVKEKATGRYYAMKILKKEVIVAKDEVAHTLTENRVLQNSRHPFLTALKYS FQTHDRLCFVMEYANGGELFFHLSRERVFSEDRARFYGAEIVSALDYLHSEKNV VYRDLKLENLMLDKDGHIKITDFGLCKEGIKDGATMKTFCGTPEYLAPEVLEDN DYGRAVDWWGLGVVMYEMMCGRLPFYNQDHEKLFELILMEEIRFPRTLGPEAKS LLSGLLKKDPTQRLGGGSEDAKEIMQHRFFANIVWQDVYEKKLSPPFKPQVTSE TDTRYFDEEFTAQMITITPPDQDDSMECVDSERRPHFPQFSYSASGTA
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分子量
59.1kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Identification
Protein Description
AKT1, a pivotal serine/threonine kinase, plays a crucial role in various cellular processes, including metabolism, growth, survival, and proliferation, making it a key player in the PI3K/Akt signaling pathway. Aberrant activation of AKT1 has been implicated in numerous human diseases, particularly cancer, as it promotes tumor cell growth and resistance to apoptosis. Research on AKT1 recombinant protein has gained significant attention due to its potential as a therapeutic target. Understanding the structure and function of AKT1 through recombinant protein studies can aid in the development of specific inhibitors, which could enhance treatment strategies for cancer and metabolic disorders. Furthermore, recombinant AKT1 can be utilized in high-throughput screening of drug candidates and in mechanistic studies to dissect its role in signaling pathways. While much has been elucidated about AKT1's functions, ongoing research aims to explore its interactions with various substrates and regulatory proteins, emphasizing the protein’s relevance in both normal physiology and pathological conditions. Thus, the production and characterization of AKT1 recombinant protein are vital for advancing our knowledge of its biological functions and therapeutic applications.
