Cat: PA1000-8952

Recombinant Human VEGF145 Protein,His

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Analytical Data

  • Gene name

    VEGF145

  • Application

    SPRMSTBLIITCELISACELL ASSAYDRUG SCREENING

  • Alternative Names

    VEGF145;VEGF;Vascular endothelial growth factor A. long form

  • Species

    Human

  • Source

    E. coli

  • Tag

    His tag N-Terminus

  • Purity

    Greater than 90% as determined by SDS-PAGE.

  • Uniprot

    P15692

  • Expression Region

    1-395aa

  • AA Sequence

    MTDRQTDTAPSPSYHLLPGRRRTVDAAASRGQGPEPAPGGGVEGVGARGVALKLFVQLLGCSRFGGAVVRAGEAEPSGAARSASSGREEPQPEEGEEEEEKEEERGPQWRLGARKPGSWTGEAAVCADSAPAARAPQALARASGRGGRVARRGAEESGPPHSPSRRGSASRAGPGRASETMNFLLSWVHWSLALLLYLHHAKWSQAAPMAEGGGQNHHEVVKFMDVYQRSYCHPIETLVDIFQEYPDEIEYIFKPSCVPLMRCGGCCNDEGLECVPTEESNITMQIMRIKPHQGQHIGEMSFLQHNKCECRPKKDRARQEKKSVRGKGKGQKRKRKKSRYKSWSVPCGPCSERRKHLFVQDPQTCKCSCKNTDSRCKARQLELNERTCRCDKPRR

  • Molecular Weight

    43.5 kDa

  • Endotoxin

    < 1.0 EU per μg protein as determined by the LAL method.

  • Form

    Freeze-dried powder

  • Buffer formulation

    PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

  • Reconstitution

    Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

  • Customization

    Site-directed mutagenesis Custom tag design Custom buffer formulation Custom full-length protein production

  • Stability Test

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.

  • Storage & Shelf Life

    Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

  • Shipping

    In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.

Quality inspection process

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Protein Description

Vascular Endothelial Growth Factor (VEGF) plays a crucial role in angiogenesis, the process of forming new blood vessels, which is essential for normal development and healing, as well as for pathological conditions such as cancer. Among the various isoforms of VEGF, VEGF145, a variant that is generated through alternative splicing, has gained attention due to its unique properties and potential therapeutic applications. Research has shown that VEGF145 exhibits distinct biological activities compared to other isoforms, which may influence its role in vascular permeability and endothelial cell function. Understanding the specific functions of VEGF145 could provide insights into targeted therapies for diseases characterized by abnormal angiogenesis, such as tumors or diabetic retinopathy. The recombination of VEGF145 for experimental purposes enables the study of its effects in vitro and in vivo, offering a platform to explore its mechanisms and interactions with other cellular factors. This research is critical for developing innovative therapeutic strategies aimed at modulating angiogenesis, ultimately improving outcomes in various diseases linked to abnormal blood vessel growth.

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