Recombinant Mouse IL-1 alpha Protein

IL-1 alpha

IL-1 alpha is a ubiquitous and pivotal pro-inflammatory cytokine. IL-1 alpha is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury, and thus induces apoptosis. IL-1 alpha plays an important role in inflammation and bridges the innate and adaptive immune systems. IL-1 alpha mediates the activation of NF-kappa-B and the three MAPK pathways p38, p42/p44 and JNK pathways[1][2]. IL-1 alpha protein, Mouse is a recombinant protein consisting of 156 amino acids (S115-A270) and is produced by E. coli.

SPRMSTBLIITCELISACELL ASSAYDRUG SCREENING

1. The ED50 is <2>5.0 × 108 units/mg. 2. Measured in a proliferation assay using CTLL-2 Mouse T lymphocytes cell. The ED50 for this effect is 0.5597 pg/mL, corresponding to a specific activity is 1.787×109 units/mg. 3. Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. The ED50 for this effect is 0.935 pg/mL, corresponding to a specific activity is 1.07×109 units/mg. Measured in a proliferation assay using CTLL-2 Mouse T lymphocytes cell. The ED50 for this effect is 0.5597pg/mL, corresponding to a specific activity is 1.787×109 units/mg.

rMuIL-1α; Hematopoietin-1; IL1A; IL1F1; IL-1 alpha

Mouse

E. coli

Tag Free

Greater than 95% as determined by reducing SDS-PAGE. as determined by SDS-PAGE.

P01582

S115-S270

Full Length of Mature Protein

16 kDa

< 1.0 EU per μg protein as determined by the LAL method.

Freeze-dried powder

PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

Site-directed mutagenesis Custom tag design Custom buffer formulation Custom full-length protein production

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.