Analytical Data
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基因名
ARF1
- Application
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别名
ARF1；ADP-ribosylation factor 1
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P84077
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表达区间
2-181aa
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氨基酸序列
GNIFANLFKGLFGKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGF NVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRERVN EAREELMRMLAEDELRDAVLLVFANKQDLPNAMNAAEITDKLGLHSLRHR NWYIQATCATSGDGLYEGLDWLSNQLRNQK
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分子量
21 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
ARF1 (ADP-ribosylation factor 1) is a member of the ARF family of small GTPases, playing a crucial role in intracellular membrane trafficking and cytoskeletal dynamics. It is particularly involved in the formation of COPI-coated vesicles, which are essential for retrograde transport from the Golgi apparatus to the endoplasmic reticulum. The regulation of ARF1 activity, through GDP-GTP cycling, is vital for maintaining cellular homeostasis and facilitating various cellular processes, including endocytosis and exocytosis. Research on ARF1 has gained traction due to its implications in various diseases, including cancer and neurodegenerative disorders, where dysregulation of membrane trafficking is often observed. The recombination and characterization of ARF1 proteins have enabled scientists to dissect its functional mechanisms and interactions with downstream effectors. Utilizing recombinant ARF1 proteins allows for the exploration of its GTPase activity, binding partners, and the biochemical pathways it influences. As a result, the study of ARF1 has provided insights into fundamental cellular processes and highlighted its potential as a therapeutic target in diseases linked to membrane trafficking anomalies. Understanding ARF1's role and regulation offers promising avenues for developing strategies to manipulate this pathway for therapeutic benefits.
