Analytical Data
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Gene name
dnapkcs
- Application
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Alternative Names
dnapkcs; DDB_G0281167; DNA-dependent protein kinase catalytic subunit; DNA-PK catalytic subunit; DNA-PKcs; EC 2.7.11.1
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Species
Dictyostelium discoideum
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Source
E. coli
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Tag
N- His & C- Myc
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q54UC0
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Expression Region
3910-4250aa
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Molecular Weight
46.1 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
DNA-dependent protein kinase (DNA-PK), a crucial component of the non-homologous end joining (NHEJ) pathway, plays a vital role in the repair of DNA double-strand breaks. The DNA-PK complex comprises the catalytic subunit DNA-PKcs and the Ku heterodimer, which recognizes DNA ends and facilitates the recruitment of additional repair proteins. Dysregulation or deficiency of DNA-PK activity can lead to genomic instability and contribute to various diseases, including cancer and neurodegenerative disorders. Consequently, the study of DNA-PKcs as a recombinant protein has gained prominence in molecular biology and therapeutic research. By producing and characterizing recombinant DNA-PKcs, researchers aim to elucidate its structural and functional properties, understand its role in DNA repair mechanisms, and explore its potential as a target for cancer therapies. The recombinant protein allows for detailed studies on the kinase's activity, interactions with DNA and other repair factors, and the development of small molecule inhibitors that could enhance the efficacy of existing chemotherapeutic agents. Understanding the mechanisms governing DNA-PKcs activity paves the way for novel therapeutic strategies aimed at improving treatment outcomes for cancers characterized by DNA repair deficiencies.
