Analytical Data
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基因名
dnapkcs
- Application
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别名
dnapkcs; DDB_G0281167; DNA-dependent protein kinase catalytic subunit; DNA-PK catalytic subunit; DNA-PKcs; EC 2.7.11.1
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种属
Dictyostelium discoideum
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表达系统
E. coli
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标签
N- His & C- Myc
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q54UC0
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表达区间
3910-4250aa
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分子量
46.1 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
DNA-dependent protein kinase (DNA-PK), a crucial component of the non-homologous end joining (NHEJ) pathway, plays a vital role in the repair of DNA double-strand breaks. The DNA-PK complex comprises the catalytic subunit DNA-PKcs and the Ku heterodimer, which recognizes DNA ends and facilitates the recruitment of additional repair proteins. Dysregulation or deficiency of DNA-PK activity can lead to genomic instability and contribute to various diseases, including cancer and neurodegenerative disorders. Consequently, the study of DNA-PKcs as a recombinant protein has gained prominence in molecular biology and therapeutic research. By producing and characterizing recombinant DNA-PKcs, researchers aim to elucidate its structural and functional properties, understand its role in DNA repair mechanisms, and explore its potential as a target for cancer therapies. The recombinant protein allows for detailed studies on the kinase's activity, interactions with DNA and other repair factors, and the development of small molecule inhibitors that could enhance the efficacy of existing chemotherapeutic agents. Understanding the mechanisms governing DNA-PKcs activity paves the way for novel therapeutic strategies aimed at improving treatment outcomes for cancers characterized by DNA repair deficiencies.
