Analytical Data
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Gene name
CFL1
- Application
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Alternative Names
CFL1；CFL;Cofilin-1
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P23528
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Expression Region
2-166aa
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AA Sequence
ASGVAVSDG VIKVFNDMKV RKSSTPEEVK KRKKAVLFCL SEDKKNIILE EGKEILVGDV GQTVDDPYAT FVKMLPDKDC RYALYDATYE TKESKKEDLV FIFWAPESAP LKSKMIYASS KDAIKKKLTG IKHELQANCY EEVKDRCTLA EKLGGSAVIS LEGKPL
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
CFL1 (Cofilin 1) is an actin-binding protein that plays a crucial role in the regulation of the cytoskeleton, which is essential for various cellular processes, including cell motility, division, and shape maintenance. Research has shown that CFL1 is involved in the disassembly of actin filaments, facilitating the dynamic reorganization of the cytoskeleton in response to extracellular signals. Dysregulation of CFL1 has been linked to several diseases, including cancer, where its altered function can affect cell migration and invasion, supporting tumor metastasis. Additionally, CFL1 has been implicated in neurodegenerative disorders and other conditions where actin dynamics are compromised. As a result, understanding the structure, function, and regulation of CFL1 is critical for uncovering its role in normal physiology and disease pathology. The development and characterization of recombinant CFL1 proteins have enabled researchers to gain insights into its biochemical properties and mechanism of action, paving the way for potential therapeutic interventions that target CFL1 signaling pathways. Furthermore, studying CFL1 in various model systems allows for the exploration of its interactions with other proteins and its impact on cellular behavior, thus providing a comprehensive framework for investigating its functions in health and disease.
