Analytical Data
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Gene name
PF-4/CXCL4
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简介
PF4V1, also known as CXCL4L1, is a non-allelic variant of CXCL4. PF4V1 is a platelet-associated chemokine, and it binds to heparin is much weaker than in the close homolog PF4/CXCL4. PF4V1 is an inhibitor of angiogenesis and inhibitor of endothelial cell chemotaxis. PF4V1 is involved in inflammation, angiogenesis, and cancer[1][2]. PF4V1 Protein, Human (HEK293, Fc) is produced in HEK293 cells with a C-Terminal Fc-tag. It consists of 104 amino acids (M1-S104).
- Application
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Biological Activity
Measured by its ability to inhibit proliferation of HUVEC human umbilical vein endothelial cells. The ED50 of this effect is 37.49 ng/mL, corresponding to a specific activity is 2.67×104 units/mg. Measured by its ability to inhibit proliferation of HUVEC human umbilical vein endothelial cells. The ED50 of this effect is 37.49 ng/ml, corresponding to a specific activity is 2.67×104 units/mg.
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Alternative Names
Platelet factor 4 variant; CXCL4L1; PF4var1; SCYB4V1
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Species
Human
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Source
HEK293
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Tag
C-mFc
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Purity
Greater than 95% as determined by reducing SDS-PAGE.
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Uniprot
NP_002611.1
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Expression Region
F31-S104
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Protein Length
Full Length of Mature Protein
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Molecular Weight
38.7 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
PF-4, also known as CXCL4, is a chemokine produced primarily by activated platelets, playing a critical role in inflammation and immune responses. Its involvement in various pathological conditions, including cardiovascular diseases, cancer, and thrombosis, has garnered significant research interest. PF-4 acts by binding to specific receptors on target cells, influencing cell migration, and modulating angiogenesis. The study of recombinant PF-4/CXCL4 proteins is pivotal for understanding its biological functions and therapeutic potential. By producing recombinant forms, researchers can manipulate its structure and evaluate its effects in vitro and in vivo. This recombinant protein serves as a valuable tool for elucidating the mechanisms underlying PF-4's actions and exploring its applications in clinical settings, such as treating inflammatory diseases or enhancing wound healing. Furthermore, the investigation of PF-4/CXCL4's role in tumor microenvironments has opened avenues for targeted cancer therapies, highlighting its significance in current biomedical research. Overall, the exploration of PF-4/CXCL4 recombinant proteins is essential for advancing our knowledge of chemokine biology and developing innovative therapeutic strategies.
