Analytical Data
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Gene name
VGLUT1
- Application
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Alternative Names
VGLUT1；BNPI；VGLUT1；Vesicular glutamate transporter 1
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9P2U7
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Expression Region
1-560aa
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AA Sequence
MEFRQEEFRKLAGRALGKLHRLLEKRQEGAETLELSADGRPVTTQTRDPP VVDCTCFGLPRRYIIAIMSGLGFCISFGIRCNLGVAIVSMVNNSTTHRGG HVVVQKAQFSWDPETVGLIHGSFFWGYIVTQIPGGFICQKFAANRVFGFA IVATSTLNMLIPSAARVHYGCVIFVRILQGLVEGVTYPACHGIWSKWAPP LERSRLATTAFCGSYAGAVVAMPLAGVLVQYSGWSSVFYVYGSFGIFWYL FWLLVSYESPALHPSISEEERKYIEDAIGESAKLMNPLTKFSTPWRRFFT SMPVYAIIVANFCRSWTFYLLLISQPAYFEEVFGFEISKVGLVSALPHLV MTIIVPIGGQIADFLRSRRIMSTTNVRKLMNCGGFGMEATLLLVVGYSHS KGVAISFLVLAVGFSGFAISGFNVNHLDIARRYASILMGISNGVGTLSGM VCPIIVGAMTKHKTREEWQYVFLIASLVHYGGVIFYGVFASGEKQPWAEP EEMSEEKCGFVGHDQLAGSDDSEMEDEAEPPGAPPAPPPSYGATHSTFQP PRPPPPVRDY
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
VGLUT1 (vesicular glutamate transporter 1) is a crucial protein involved in the packaging of glutamate, the primary excitatory neurotransmitter in the central nervous system, into synaptic vesicles. Understanding VGLUT1 is vital due to its role in synaptic transmission and plasticity, which are essential for cognitive functions such as learning and memory. Dysregulation of VGLUT1 has been implicated in various neurological disorders, including anxiety, depression, and schizophrenia, making it a significant target for therapeutic interventions. Research on recombinant VGLUT1 protein aims to elucidate its structure-function relationship and regulatory mechanisms, enabling the identification of potential pharmacological targets. Advancements in producing recombinant VGLUT1 have provided insights into its biochemical properties, interaction with other proteins, and the molecular dynamics of glutamate transport. Investigating the recombinant version of this protein in various cellular models can enhance our understanding of synaptic mechanisms and pave the way for developing novel strategies to modulate glutamatergic signaling in disease contexts, ultimately contributing to the advancement of neuropharmacology.
