Analytical Data
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Gene name
Hmgcs1
- Application
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Alternative Names
Hmgcs1；HMGCS；Hydroxymethylglutaryl-CoA synthase. cytoplasmic
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q01581
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Expression Region
1-520aa
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AA Sequence
MGSSHHHHHH SSGLVPRGSH MGSMPGSLPL NAEACWPKDV GIVALEIYFP SQYVDQAELE KYDGVDAGKY TIGLGQAKMG FCTDREDINS LCMTVVQNLM ERNNLSYDCI GRLEVGTETI IDKSKSVKTN LMQLFEESGN TDIEGIDTTN ACYGGTAAVF NAVNWIESSS WDGRYALVVA GDIAVYATGN ARPTGGVGAV ALLIGPNAPL IFERGLRGTH MQHAYDFYKP DMLSEYPIVD GKLSIQCYLS ALDRCYSVYC KKIHAQWQKE GNDKDFTLND FGFMIFHSPY CKLVQKSLAR MLLNDFLNDQ NRDKNSIYSG LEAFGDVKLE DTYFDRDVEK AFMKASSELF SQKTKASLLV SNQNGNMYTS SVYGSLASVL AQYSPQQLAG KRIGVFSYGS GLAATLYSLK VTQDATPGSA LDKITASLCD LKSRLDSRTG VAPDVFAENM KLREDTHHLV NYIPQGSIDS LFEGTWYLVR VDEKHRRTYA RRPTPNDDTL DEGVGLVHSN IATEHIPSPA KKVPRLPATA AEPEAAVISN GEH
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Molecular Weight
60 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
HMGCS1, or 3-hydroxy-3-methylglutaryl-CoA synthase 1, is a key enzyme in the mevalonate pathway, which is crucial for the biosynthesis of sterols and isoprenoids. This enzyme catalyzes the condensation of acetoacetyl-CoA and acetyl-CoA to form HMG-CoA, an important intermediate in cholesterol synthesis and cellular metabolism. Given its central role in lipid biosynthesis, HMGCS1 has been implicated in various metabolic disorders, including cardiovascular diseases and cancer, making it a significant target for therapeutic intervention. Recent studies have focused on the recombinant production of HMGCS1 protein to facilitate functional assays and structural analysis. By using recombinant DNA technology, researchers can produce large quantities of HMGCS1, enabling detailed investigations into its enzymatic activity, regulation, and role in disease. Moreover, understanding the three-dimensional structure of HMGCS1, obtained through techniques such as X-ray crystallography or cryo-electron microscopy, can provide insights into its interaction with potential inhibitors or substrates. This research not only enhances our understanding of the enzyme's biological functions but also aids in the development of novel drugs that could modulate its activity in pathological conditions. Overall, the investigation of HMGCS1 recombinant protein is essential for advancing our knowledge of lipid metabolism and its implications for human health.
