Analytical Data
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Gene name
HTRA1
- Application
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Alternative Names
HTRA1；HTRA；PRSS11；Serine protease HTRA1
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q92743
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Expression Region
23-480aa
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AA Sequence
QLSRAGRSAPLAAGCPDRCEPARCPPQPEHCEGGRARDACGCCEVCGAPE GAACGLQEGPCGEGLQCVVPFGVPASATVRRRAQAGLCVCASSEPVCGSD ANTYANLCQLRAASRRSERLHRPPVIVLQRGACGQGQEDPNSLRHKYNFI ADVVEKIAPAVVHIELFRKLPFSKREVPVASGSGFIVSEDGLIVTNAHVV TNKHRVKVELKNGATYEAKIKDVDEKADIALIKIDHQGKLPVLLLGRSSE LRPGEFVVAIGSPFSLQNTVTTGIVSTTQRGGKELGLRNSDMDYIQTDAI INYGNSGGPLVNLDGEVIGINTLKVTAGISFAIPSDKIKKFLTESHDRQA KGKAITKKKYIGIRMMSLTSSKAKELKDRHRDFPDVISGAYIIEVIPDTP AEAGGLKENDVIISINGQSVVSANDVSDVIKRESTLNMVVRRGNEDIMIT VIPEEIDP
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Molecular Weight
50 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
HTRA1 (High-temperature requirement A serine peptidase 1) is a serine protease that has garnered significant attention in biomedical research due to its involvement in various pathological processes, particularly in age-related diseases such as age-related macular degeneration (AMD). This extracellular enzyme is known to play a crucial role in the regulation of cellular processes, including protein quality control, apoptosis, and extracellular matrix remodeling. Abnormal HTRA1 expression has been linked to increased susceptibility to AMD, making it a potential biomarker and therapeutic target for this vision-threatening condition. Additionally, HTRA1 is involved in neurodegenerative diseases and certain cancers, further underscoring its importance in human health. The recombinant form of HTRA1 is particularly useful for detailed biochemical and structural studies, allowing researchers to dissect its functional mechanisms, enzyme activity, and interaction with substrates and inhibitors. The production of HTRA1 recombinant protein enables high-throughput screening and the development of novel therapeutic strategies, including small molecules or monoclonal antibodies that target the dysregulated pathways associated with its aberrant expression. Overall, HTRA1 recombinant protein research highlights its significance not only in understanding fundamental biological processes but also in identifying innovative approaches to disease treatment and prevention.
