Recombinant Human Ephrin-A1/EFNA1 Protein(HEK293), C-hFc

Ephrin-A1/EFNA1

The Ephrin-A1/EFNA1 protein is a GPI-binding ligand that interacts with Eph receptors and is critical for migration and adhesion. It recruits VAV2, VAV3, and PI3 kinase p85 subunits through EPHA2 phosphorylation, activating RAC1 GTPase for endothelial cell migration. Ephrin-A1/EFNA1 Protein, Human (HEK293, Fc) is the recombinant human-derived Ephrin-A1/EFNA1 protein, expressed by HEK293 , with C-hFc labeled tag.

SPRMSTBLIITCELISACELL ASSAYDRUG SCREENING

Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human Ephrin-A1 (HY-P72662) is immobilized at 2 µg/mL (100 µL/well) can bind Human EphA1 (HY-P70321). The ED50 for this effect is 17.1 ng/mL. Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human Ephrin-A1 (HY-P72662) is immobilized at 2 µg/mL (100 µL/well) can bind Human EphA1 (HY-P70321). The ED50 for this effect is 17.1 ng/mL.

Ephrin-A1; LERK-1; EFNA1; EPLG1; TNFAIP4

Human

HEK293

C-hFc

Greater than 95% as determined by reducing SDS-PAGE.

P20827-1

D19-S182

Full Length of Mature Protein

55-60 kDa

< 1.0 EU per μg protein as determined by the LAL method.

Freeze-dried powder

PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

Site-directed mutagenesis Custom tag design Custom buffer formulation Custom full-length protein production

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.