Analytical Data
-
Gene name
KRTHA4
- Application
-
Alternative Names
Ha 4; HA4; Hair keratin; Hair keratin; type I Ha4; Hard keratin type I 4; HHA4; HKA4; K34; Keratin 34 ; Keratin; Keratin; hair; acidic; 4; Keratin; type I cuticular Ha4; Keratin-34; KRT34; KRT34_HUMAN; KRTHA4; type I cuticular Ha4; type I Ha4
-
Species
Human
-
Source
E. coli
-
Tag
GST-tag at N-terminal
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
O76011
-
Expression Region
1-436aa
-
AA Sequence
MLYAKPPPTINGIKGLQRKERLKPAHIHLQQLTCFSITCSSTMSYSCCLPSLGCRTSCSSRPCVPPSCHGYTLPGACNIPANVSNCNWFCEGSFNGSEKETMQFLNDRLASYLEKVRQLERDNAELEKLIQERSQQQEPLLCPSYQSYFKTIEELQQKILCAKAENARLVVNIDNAKLASDDFRSKYQTEQSLRLLVESDINSIRRILDELTLCKSDLESQVESLREELICLKKNHEEEVNTLRSQLGDRLNVEVDTAPTVDLNQVLNETRSQYEALVETNRREVEQWFATQTEELNKQVVSSSEQLQSCQAEIIELRRTVNALEIELQAQHNLRDSLENTLTESEAHYSSQLSQVQSLITNVESQLAEIRCDLERQNQEYQVLLDVRARLECEINTYRSLLESEDCKLPCNPCATTNASGNSCGPCGTSQKGCCN
-
Molecular Weight
75.8 kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
KRTHA4, also known as Keratin 17, is a type of intermediate filament protein primarily expressed in epithelial tissues and is considered a member of the keratin family. Its expression is particularly prominent during wound healing and in various pathological conditions, including certain skin disorders. Research has indicated that KRTHA4 plays a crucial role in cellular proliferation, differentiation, and response to stress, suggesting its involvement in maintaining epithelial integrity and function. The significance of KRTHA4 in both normal physiology and disease states has led to an increased interest in understanding its structure and function. Advanced techniques such as recombinant protein expression and purification have enabled researchers to study KRTHA4 at the molecular level, providing insights into its interactions with other cellular components and its impact on cellular signaling pathways. Additionally, alterations in KRTHA4 expression have been correlated with various cancers, positioning it as a potential biomarker for diagnosis and therapy. Investigating the structural characteristics of KRTHA4, including its role in cytoskeletal organization and mechanical resilience of epithelial tissues, can contribute to a better understanding of its biological functions and its potential therapeutic applications in regenerative medicine and cancer treatment. Thus, research on the recombinant protein KRTHA4 not only expands our knowledge of keratin biology but also opens avenues for innovative therapeutic strategies targeting epithelial-related diseases.
