Analytical Data
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Gene name
EphA2
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简介
EphA2 protein is a receptor tyrosine kinase that promiscuously binds to ephrin A ligands to initiate bidirectional signaling. EphA2 is activated by ephrin-A1/EFNA1, regulates cell migration, adhesion, proliferation and differentiation, modulates DSG1 and inhibits ERK1/ERK2 signaling. EphA2 Protein, Mouse (HEK293, His) is the recombinant mouse-derived EphA2 protein, expressed by HEK293 , with C-His labeled tag.
- Application
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Biological Activity
Measured by its binding ability in a functional ELISA. Immobilized mouse EphA2 at 2 μg/mL (100 μl/well) can bind mouse EphrinA1 with a linear range of 0.16-20 ng/mL. Immobilized Recombinant Mouse EphA2 Proteinat 2 μg/mL (100 μL/well) can bind Recombinant Mouse Ephrin-A1 / EFNA1 Protein, the ED50 is 11.4 ng/mL.
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Alternative Names
Ephrin type-A receptor 2; Epithelial cell kinase; EPHA2; ECK
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Species
Mouse
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Source
HEK293
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Tag
C-His
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Purity
Greater than 95% as determined by reducing SDS-PAGE.
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Uniprot
Q03145
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Expression Region
K26-N535
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AA Sequence
KEVVLLDFAAMKGELGWLTHPYGKGWDLMQNIMDDMPIYMYSVCNVVSGDQDNWLRTNWVYREEAERIFIELKFTVRDCNSFPGGASSCKETFNLYYAESDVDYGTNFQKRQFTKIDTIAPDEITVSSDFEARNVKLNVEERMVGPLTRKGFYLAFQDIGACVALLSVRVYYKKCPEMLQSLARFPETIAVAVSDTQPLATVAGTCVDHAVVPYGGEGPLMHCTVDGEWLVPIGQCLCQEGYEKVEDACRACSPGFFKSEASESPCLECPEHTLPSTEGATSCQCEEGYFRAPEDPLSMSCTRPPSAPNYLTAIGMGAKVELRWTAPKDTGGRQDIVYSVTCEQCWPESGECGPCEASVRYSEPPHALTRTSVTVSDLEPHMNYTFAVEARNGVSGLVTSRSFRTASVSINQTEPPKVRLEDRSTTSLSVTWSIPVSQQSRVWKYEVTYRKKGDANSYNVRRTEGFSVTLDDLAPDTTYLVQVQALTQEGQGAGSKVHEFQTLSTEGSAN
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Protein Length
Extracellular Domain
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Molecular Weight
65-95 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
EphA2 is a member of the Eph receptor tyrosine kinase family, which plays a crucial role in various biological processes, including cell adhesion, migration, and differentiation. This receptor is predominantly expressed in epithelial tissues and is implicated in several pathological conditions, such as cancer progression and metastasis. Elevated levels of EphA2 have been associated with aggressive tumor phenotypes, making it a potential biomarker for cancer diagnosis and a target for therapeutic intervention. The study of EphA2 recombinant proteins provides valuable insights into its structural and functional characteristics, enabling researchers to investigate its signaling pathways and interactions with ligands, particularly the ephrin family. Understanding these mechanisms is essential for developing EphA2-targeted therapies, particularly in cancers where its expression is dysregulated. Furthermore, recombinant EphA2 proteins can be used in various experimental applications, including drug screening and the study of cell signaling dynamics. By elucidating the role of EphA2 in tumor biology, researchers aim to uncover novel strategies for cancer treatment and improve clinical outcomes for patients.
