Analytical Data
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Gene name
OTOF
- Application
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Alternative Names
OTOF；FER1L2；Otoferlin
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9HC10
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Expression Region
1461-1868aa
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AA Sequence
KVPLPEDVSREAGYDSTYGMFQGIPSNDPINVLVRVYVVRATDLHPADINGKADPYIAIRLGKTDIRDKENYISKQLNPVFGKSFDIEASFPMESMLTVAVYDWDLVGTDDLIGETKIDLENRFYSKHRATCGIAQTYSTHGYNIWRDPMKPSQILTRLCKDGKVDGPHFGPPGRVKVANRVFTGPSEIEDENGQRKPTDEHVALLALRHWEDIPRAGCRLVPEHVETRPLLNPDKPGIEQGRLELWVDMFPMDMPAPGTPLDISPRKPKKYELRVIIWNTDEVVLEDDDFFTGEKSSDIFVRGWLKGQQEDKQDTDVHYHSLTGEGNFNWRYLFPFDYLAAEEKIVISKKESMFSWDETEYKIPARLTLQIWDADHFSADDFLGAIELDLNRFPRGAKTAKQCTMEM
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Molecular Weight
52.6 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
OTOF (Otoferlin) is a crucial protein that plays a significant role in auditory signaling and is primarily expressed in the inner hair cells of the cochlea, which are essential for sound transduction. Mutations in the OTOF gene are linked to auditory neuropathy and profound hearing loss, making OTOF a subject of intensive research to understand its function in auditory pathways and potential therapeutic avenues. The protein is involved in the regulation of synaptic vesicle fusion and neurotransmitter release at the ribbon synapses of inner hair cells, which is crucial for converting sound waves into electrical signals that the brain can interpret. Recombination and characterization of OTOF in laboratory settings not only aid in elucidating its biological functions but also facilitate the exploration of gene therapy and other innovative treatments for hearing impairments associated with OTOF dysregulation. As the understanding of OTOF's mechanistic role advances, it opens new doors for developing targeted interventions for hearing loss, thereby impacting millions affected by auditory disorders worldwide.
