Analytical Data
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基因名
plyC
- Application
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别名
plyC；Endolysin PlyC. small cell-wall binding subunit
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
B0XMA2
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表达区间
21-420aa
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氨基酸序列
LVAFPGAEGFGANAIGGRNGQVYVVTNLNDSGTGSLRDAVSATDRIVVFAVGGVIKISDRIVVSKRVTILGQTAPGDGITVYGNGWSFSNADDAIVRYIRIRMGKGGSSGKDALGIAEGNRMIFDHVSVSWGRDETFSINGDASNITVQNSIIAQGLETHSCGGLMQTDGGVSLFRNLYIDNKTRNPKVKGVNEFTNNVVYNWGGGGGYIAGDSAGQSYANIIGNYFISGPSTSVTAFTRGNANFHGYVQNNYYDPDKDGQLDGFELGVSSSNYGGVAIMSSKYNYPAVAYTMSPAEAVTYVTKYAGASKVRDSVDTQLIAQVQSWGTEGGLISDEATMGGPGTLNGGTPAKDTDGDGIPDEAEKQLGTDPNTNDSMKLHSSGYTYLEVWANSLVPSTYH
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分子量
49.5 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
PlyC is a phage lysin derived from the bacteriophage C1, known for its ability to lyse specific bacterial strains, particularly those of the Streptococcus genus. As antimicrobial resistance becomes a pressing global health issue, the quest for alternative antibacterial agents has intensified, making PlyC an attractive candidate. This enzyme exhibits a unique mechanism of action by cleaving the peptidoglycan layer of bacterial cell walls, leading to cell lysis. Unlike conventional antibiotics that may target specific bacterial pathways, PlyC shows a broad-spectrum efficacy against various pathogenic strains, including drug-resistant bacteria. Researchers have been focused on the recombinant expression of PlyC to produce it in sufficient quantities for therapeutic applications. Through genetic engineering techniques, scientists have successfully optimized the production of PlyC, enhancing its stability and activity. Studies have shown promising results in using PlyC as a potential treatment for bacterial infections in both clinical and veterinary settings. Furthermore, PlyC's ability to reduce biofilms and resistances further underscores its relevance in modern medicine. Thus, ongoing research aims to better understand PlyC's structure-function relationships, explore its synergistic effects with existing antibiotics, and develop formulations that can effectively deliver this biotherapeutic agent in clinical practice, contributing to combating multi-drug resistant infections.
