Analytical Data
-
Gene name
MAP4K3
- Application
-
Alternative Names
MAP4K3; RAB8IPL1Mitogen-activated protein kinase kinase kinase kinase 3; EC 2.7.11.1; Germinal center kinase-related protein kinase; GLK; MAPK/ERK kinase kinase kinase 3; MEK kinase kinase 3; MEKKK 3
-
Species
Human
-
Source
E. coli
-
Tag
GST-tag at N-terminal
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
Q8IVH8
-
Expression Region
1-380aa
-
AA Sequence
MNPGFDLSRRNPQEDFELIQRIGSGTYGDVYKARNVNTGELAAIKVIKLE PGEDFAVVQQEIIMMKDCKHPNIVAYFGSYLRRDKLWICMEFCGGGSLQD IYHVTGPLSELQIAYVSRETLQGLYYLHSKGKMHRDIKGANILLTDNGHV KLADFGVSAQITATIAKRKSFIGTPYWMAPEVAAVERKGGYNQLCDLWAV GITAIELAELQPPMFDLHPMRALFLMTKSNFQPPKLKDKMKWSNSFHHFV KMALTKNPKKRPTAEKLLQHPFVTQHLTRSLAIELLDKVNNPDHSTYHDF DDDDPEPLVAVPHRIHSTSRNVREEKTRSEITFGQVKFDPPLRKETEPHH ELPDSDGFLD SSEEIYYTARSNLDLQLEYG
-
Molecular Weight
70 kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
MAP4K3, a member of the MAP4K family of serine/threonine kinases, has garnered considerable attention in recent years due to its potential roles in various cellular processes, including cell proliferation, migration, and apoptosis. Initial studies highlighted its involvement in the mitogen-activated protein kinase (MAPK) signaling pathway, a critical cascade that regulates cellular responses to extracellular stimuli. Dysregulation of MAP4K3 has been implicated in numerous pathological conditions, including cancer, where it may contribute to tumor progression and metastasis. Furthermore, emerging evidence suggests that MAP4K3 interacts with different signaling networks, influencing both the immune response and cellular stress responses. This complexity underscores the importance of MAP4K3 reconstituted proteins in elucidating its biological functions and pathways. Recent research efforts have focused on characterizing MAP4K3 through recombinant expression systems, allowing investigators to dissect its enzymatic activities, interaction partners, and post-translational modifications. Moreover, understanding the regulation and functional outcomes of MAP4K3 activity may reveal novel therapeutic targets for diseases associated with its dysregulation. As such, MAP4K3 stands out as a significant focus in cellular signaling research, promising to enhance our understanding of its roles in health and disease while paving the way for potential interventions in conditions linked with its altered function.
