Analytical Data
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基因名
BCAT1
- Application
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别名
BCAT1；BCT1；ECA39；Branched-chain-amino-acid aminotransferase. cytosolic
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P54687
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表达区间
1-386aa
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氨基酸序列
MGSSHHHHHH SSGLVPRGSH MGSMKDCSNG CSAECTGEGG SKEVVGTFKA KDLIVTPATI LKEKPDPNNL VFGTVFTDHM LTVEWSSEFG WEKPHIKPLQ NLSLHPGSSA LHYAVELFEG LKAFRGVDNK IRLFQPNLNM DRMYRSAVRA TLPVFDKEEL LECIQQLVKL DQEWVPYSTS ASLYIRPTFI GTEPSLGVKK PTKALLFVLL SPVGPYFSSG TFNPVSLWAN PKYVRAWKGG TGDCKMGGNY GSSLFAQCEA VDNGCQQVLW LYGEDHQITE VGTMNLFLYW INEDGEEELA TPPLDGIILP GVTRRCILDL AHQWGEFKVS ERYLTMDDLT TALEGNRVRE MFGSGTACVV CPVSDILYKG ETIHIPTMEN GPKLASRILS KLTDIQYGRE ESDWTIVLS
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分子量
45 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
BCAT1 (branched-chain amino acid transaminase 1) is an enzyme that plays a crucial role in the metabolism of branched-chain amino acids (BCAAs), which are essential for various physiological processes, including protein synthesis, energy production, and the regulation of metabolic pathways. BCAT1 has gained substantial attention in recent years due to its association with cancer metabolism; elevated levels of BCAT1 expression have been observed in several cancer types, suggesting that it may contribute to tumor growth and survival by enhancing the availability of BCAAs for anabolic processes. Research on the recombinant protein of BCAT1 is significant as it allows for the elucidation of the enzyme's structure, function, and regulatory mechanisms. Understanding the characteristics and kinetics of BCAT1 through its recombinant form can facilitate the discovery of potential therapeutic targets, paving the way for novel cancer treatments based on the inhibition of its activity. Additionally, BCAT1's involvement in regulating neurotransmitter levels, particularly in neurological conditions, further underscores the importance of studying this enzyme. Thus, recombinant BCAT1 protein serves as a valuable tool in biochemical research, providing insights into metabolic dysregulation in cancer and other diseases, ultimately contributing to advancements in targeted therapies and personalized medicine strategies.
