Analytical Data
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基因名
MIXL1
- Application
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别名
MIXL1; MIXL; Homeobox protein MIXL1; Homeodomain protein MIX; hMix; MIX1 homeobox-like protein 1; Mix.1 homeobox-like protein
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9H2W2
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表达区间
1-232 aa
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氨基酸序列
MATAESRALQ FAEGAAFPAY RAPHAGGALL PPPSPAAALL PAPPAGPGPA TFAGFLGRDP GPAPPPPASL GSPAPPKGAA APSASQRRKR TSFSAEQLQL LELVFRRTRY PDIHLRERLA ALTLLPESRI QVWFQNRRAK SRRQSGKSFQ PLARPEIILN HCAPGTETKC LKPQLPLEVD VNCLPEPNGV GGGISDSSSQ GQNFETCSPL SEDIGSKLDS WEEHIFSAFG NF
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分子量
24.6 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
MIXL1, or Mesoderm Induction Candidate 1, is a transcription factor crucial for early embryonic development, specifically in mesoderm formation and anterior-posterior axis specification. Initially identified in Xenopus laevis, MIXL1 is conserved across various species, underscoring its fundamental role in developmental processes. Its expression is tightly regulated during embryogenesis, and aberrations in its function can lead to severe developmental disorders. The study of recombinant MIXL1 protein is pivotal for understanding its structure-function relationships, as well as its interactions with other cellular pathways involved in embryonic development. Advances in recombinant protein production techniques have enabled researchers to produce and purify MIXL1, facilitating biochemical assays and structural analyses. This research is not only significant for basic developmental biology but may also provide insights into regenerative medicine and the molecular basis of congenital anomalies. Understanding MIXL1's mechanistic roles could potentially lead to therapeutic applications in manipulating stem cell differentiation and enhancing tissue regeneration.
