Analytical Data
-
基因名
SF3B1
- Application
-
别名
SF3B1；SAP155；Splicing factor 3B subunit 1
-
种属
Human
-
表达系统
E. coli
-
标签
His tag N-Terminus
-
纯度
Greater than 90% as determined by SDS-PAGE.
-
蛋白编号
O75533
-
表达区间
326-571aa
-
氨基酸序列
TPTPGASKRKSRWDETPASQMGGSTPVLTPGKTPIGTPAMNMATPTPGHIMSMTPEQLQAWRWEREIDERNRPLSDEELDAMFPEGYKVLPPPAGYVPIRTPARKLTATPTPLGGMTGFHMQTEDRTMKSVNDQPSGNLPFLKPDDIQYFDKLLVDVDESTLFPEEQKERKIMKLLLKIKNGTPPMRKAALRQITDKAREFGAGPLFNQILPLLMSPTLEDQERHLLVKVIDRILYKLDDLVRPYV
-
分子量
33.8 kDa
-
内毒素
< 1.0 EU per μg protein as determined by the LAL method.
-
性状
Freeze-dried powder
-
缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
-
稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
SF3B1 (Splicing Factor 3b Subunit 1) is a crucial component of the spliceosome, a large RNA-protein complex responsible for the splicing of precursor mRNA into mature mRNA. Mutations in the SF3B1 gene have been linked to various hematological malignancies, notably myelodysplastic syndromes (MDS) and acute myeloid leukemia (AML). These mutations lead to aberrant splicing events that can disrupt normal gene expression and contribute to cancer progression. The study of SF3B1 and its protein interactions is vital for understanding the molecular mechanisms underlying these diseases and may offer new therapeutic targets. Recombinant SF3B1 proteins have been utilized in biochemical assays to investigate their functional roles, interactions with other splicing factors, and effects on splicing regulation. Additionally, understanding the structural aspects of SF3B1 can facilitate the development of small molecules or inhibitors that can modulate its activity. As research progresses, elucidating the role of SF3B1 not only contributes to the understanding of cancer biology but also enhances prospects for novel treatment strategies that target splicing abnormalities in malignancies.
