Analytical Data
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基因名
NPL4
- Application
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别名
Nuclear protein localization protein 4 homolog. Protein NPL4
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种属
Human
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表达系统
E. coli
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标签
GST-tag at N-terminal
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q8TAT6
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表达区间
1-608 aa
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氨基酸序列
MAESIIIRVQSPDGVKRITATKRETAATFLKKVAKEFGFQNNGFSVYINRNKTGEITASSNKSLNLLKIKHGDLLFLFPSSLAGPSSEMETSVPPGFKVFGAPNVVEDEIDQYLSKQDGKIYRSRDPQLCRHGPLGKCVHCVPLEPFDEDYLNHLEPPVKHMSFHAYIRKLTGGADKGKFVALENISCKIKSGCEGHLPWPNGICTKCQPSAITLNRQKYRHVDNIMFENHTVADRFLDFWRKTGNQHFGYLYGRYTEHKDIPLGIRAEVAAIYEPPQIGTQNSLELLEDPKAEVVDEIAAKLGLRKVGWIFTDLVSEDTRKGTVRYSRNKDTYFLSSEECITAGDFQNKHPNMCRLSPDGHFGSKFVTAVATGGPDNQVHFEGYQVSNQCMALVRDECLLPCKDAPELGYAKESSSEQYVPDVFYKDVDKFGNEITQLARPLPVEYLIIDITTTFPKDPVYTFSISQNPFPIENRDVLGETQDFHSLATYLSQNTSSVFLDTISDFHLLLFLVTNEVMPLQDSISLLLEAVRTRNEELAQTWKRSEQWATIEQLCSTVGGQLPGLHEYGAVGGSTHTATAAMWACQHCTFMNQPGTGHCEMCSLPRT
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分子量
94.5 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
NPL4 (NEDD4 family interacting protein 4) is a key player within the ubiquitin-proteasome system, playing a crucial role in the regulation of protein degradation, cell signaling, and various cellular processes. Its function is particularly vital in the context of cellular stress responses and protein quality control. The study of NPL4 has gained momentum due to its involvement in various pathological conditions, including neurodegenerative diseases and cancer, where aberrations in protein homeostasis are prevalent. Researchers have focused on understanding the structural and functional properties of NPL4, particularly in its role as a cofactor for the E3 ubiquitin ligase, which facilitates the attachment of ubiquitin molecules to target proteins for degradation. This process is essential for maintaining cellular homeostasis and preventing the accumulation of damaged or misfolded proteins. Moreover, the reengineering of NPL4 to enhance its stability or alter its interaction with other proteins is being explored as a potential therapeutic strategy. By dissecting the mechanisms of NPL4’s action and its interactions at the molecular level, scientists aim to uncover novel insights into the regulation of protein turnover and its implications in disease, paving the way for innovative approaches in drug design and therapeutic interventions. The ongoing research on NPL4 and its restructured variants holds promise in elucidating new pathways for therapeutic adaptation in diseases characterized by protein mismanagement and could provide a foundation for developing targeted therapies that restore normal cellular functions.
