Analytical Data
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基因名
AKAP14
- Application
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别名
AKAP14; AKAP28A-kinase anchor Protein 14; AKAP-14
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q86UN6
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表达区间
1-197aa
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氨基酸序列
MSETQNSTSQ KAMDEDNKAA SQTMPNTQDK NYEDELTQVA LALVEDVINY AVKIVEEERN PLKNIKWMTH GEFTVEKGLK QIDEYFSKCV SKKCWAHGVE FVERKDLIHS FLYIYYVHWS ISTADLPVAR ISAGTYFTMK VSKTKPPDAP IVVSYVGDHQ ALVHRPGMVR FRENWQKNLT DAKYSFMESF PFLFNRV
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分子量
22.8 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
AKAP14 (A-Kinase Anchoring Protein 14) is a member of the AKAP family, which serves as important scaffolding proteins that localize protein kinase A (PKA) and other signaling molecules to specific subcellular compartments. While the role of AKAPs in cellular signaling is well established, the precise function of AKAP14 remains less understood, particularly in relation to its implications in various physiological and pathological processes. Research has indicated that AKAP14 is involved in the regulation of cardiac function, neuronal signaling, and the response to stress, thereby highlighting its potential significance in cardiovascular diseases and neurodegenerative disorders. Recombination and expression of AKAP14 as a recombinant protein are critical steps in elucidating its functional properties and interactions with other signaling proteins. Investigating AKAP14's structure and dynamics can lead to a deeper understanding of its role in compartmentalized signaling and may unveil novel therapeutic targets. Additionally, the generation of recombinant AKAP14 can facilitate the development of specific inhibitors or modulators to influence its activity, paving the way for innovative strategies in the treatment of conditions associated with dysregulated PKA signaling. Overall, the study of AKAP14 recombinant protein is pivotal for advancing our knowledge of cellular signaling networks and their implications in health and disease.












