Analytical Data
-
Gene name
MBD1
- Application
-
Alternative Names
MBD1；CXXC3；PCM1；Methyl-CpG-binding domain Protein 1
-
Species
Human
-
Source
E. coli
-
Tag
His tag N-Terminus
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
Q9UIS9
-
Expression Region
1-605aa
-
AA Sequence
MAEDWLDCPALGPGWKRREVFRKSGATCGRSDTYYQSPTGDRIRSKVELT RYLGPACDLTLFDFKQGILCYPAPKAHPVAVASKKRKKPSRPAKTRKRQV GPQSGEVRKEAPRDETKADTDTAPASFPAPGCCENCGISFSGDGTQRQRL KTLCKDCRAQRIAFNREQRMFKRVGCGECAACQVTEDCGACSTCLLQLPH DVASGLFCKCERRRCLRIVERSRGCGVCRGCQTQEDCGHCPICLRPPRPG LRRQWKCVQRRCLRGKHARRKGGCDSKMAARRRPGAQPLPPPPPSQSPEP TEPHPRALAPSPPAEFIYYCVDEDELQPYTNRRQNRKCGACAACLRRMDC GRCDFCCDKPKFGGSNQKRQKCRWRQCLQFAMKRLLPSVWSESEDGAGSP PPYRRRKRPSSARRHHLGPTLKPTLATRTAQPDHTQAPTKQEAGGGFVLP PPGTDLVFLREGASSPVQVPGPVAASTEALLQEAQCSGLSWVVALPQVKQ EKADTQDEWTPGTAVLTSPVLVPGCPSKAVDPGLPSVKQEPPDPEEDKEE NKDDSASKLAPEEEAGGAGTPVITEIFSLGGTRFRDTAVWLPRSKDLKKP GARKQ
-
Molecular Weight
93 kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
MBD1 (Methyl-CpG Binding Domain Protein 1) is a crucial member of the MBD protein family, known for its role in interpreting DNA methylation marks and regulating gene expression. This protein is characterized by its ability to bind specifically to methylated cytosines in DNA, thus playing a significant role in the epigenetic regulation of gene silencing. Research has shown that MBD1 is involved in several biological processes, including embryonic development, cellular differentiation, and repression of transposable elements. In cancer biology, altered expression of MBD1 has been implicated in the promotion of tumorigenesis, thereby highlighting its potential as a biomarker or therapeutic target. The development of recombinant MBD1 protein has facilitated studies aimed at elucidating its function, interactions, and mechanisms of action within the epigenetic landscape. Understanding the structural features and binding affinities of MBD1 not only enhances our knowledge of epigenetic regulation but also paves the way for developing novel strategies to manipulate gene expression for therapeutic purposes. Furthermore, advancements in protein engineering techniques are enabling researchers to create modified forms of MBD1 with altered binding properties, providing deeper insights into the specificity and dynamics of methylation-dependent gene regulation. Overall, the study of recombinant MBD1 proteins is integral to dissecting the complex interplay between DNA methylation and gene expression, with broad implications for developmental biology, cancer research, and epigenetic therapy.
