Analytical Data
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基因名
ARHGEF4
- Application
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别名
ARHGEF4; KIAA1112Rho guanine nucleotide exchange factor 4
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种属
Human
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表达系统
E. coli
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标签
GST-tag at N-terminal
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9NR80
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表达区间
1-690aa
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氨基酸序列
MPWEEPAGEKPSCSHSQKAFHMEPAQKPCFTTDMVTWALLCISAETVRGEAPSQPRGIPHRSPVSVDDLWLEKTQRKKLQKQAHVERRLHIGAVHKDGVKCWRKTIITSPESLNLPRRSHPLSQSAPTGLNHMGWPEHTPGTAMPDGALDTAVCADEVGSEEDLYDDLHSSSHHYSHPGGGGEQLAINELISDGSVVCAEALWDHVTMDDQELGFKAGDVIEVMDATNREWWWGRVADGEGWFPASFVRLRVNQDEPADDDAPLAGNSGAEDGGAEAQSSKDQMRTNVINEILSTERDYIKHLRDICEGYVRQCRKRADMFSEEQLRTIFGNIEDIYRCQKAFVKALEQRFNRERPHLSELGACFLEHQADFQIYSEYCNNHPNACVELSRLTKLSKYVYFFEACRLLQKMIDISLDGFLLTPVQKICKYPLQLAELLKYTHPQHRDFKDVEAALHAMKNVAQLINERKRRLENIDKIAQWQSSIEDWEGEDLLVRSSELIYSGELTRVTQPQAKSQQRMFFLFDHQLIYCKKDLLRRDVLYYKGRLDMDGLEVVDLEDGKDRDLHVSIKNAFRLHRGATGDSHLLCTRKPEQKQRWLKAFAREREQVQLDQETGFSITELQRKQAMLNASKQQVTGKPKAVGRPCYLTRQKHPALPSNRPQQQVLVLAEPRRKPSTFWHSISRLAPFRK
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分子量
75.9 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
ARHGEF4, also known as Tiam1, is a member of the guanine nucleotide exchange factor (GEF) family that specifically activates Rho GTPases, which play critical roles in various cellular processes, including cytoskeletal dynamics, cell migration, and proliferation. The study of ARHGEF4 is particularly important due to its involvement in several pathophysiological conditions, including cancer and neurodegenerative diseases. Recent research has highlighted its potential as a therapeutic target, as aberrant Rho GTPase signaling is often associated with tumor progression and metastasis. Moreover, ARHGEF4 has been implicated in signaling pathways that regulate neuronal development and function, making it a candidate for investigating neurological disorders. Experimental studies often involve the generation of recombinant ARHGEF4 proteins to elucidate its structure-function relationships and to explore its interactions with various downstream effectors. Understanding the biochemical properties and regulatory mechanisms of ARHGEF4 can provide insights into its role in cellular signaling and its potential as a molecular target in therapeutic interventions. Consequently, research on ARHGEF4 recombinant proteins is pivotal for advancing our knowledge of Rho GTPase-related cellular functions and for developing strategies to manipulate these pathways in disease contexts.
