Analytical Data
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Gene name
ATP6V1A
- Application
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Alternative Names
70kDa subunit; ATP6A1; ATP6V1A; ATP6V1A1; ATPase H+ transporting lysosomal subunit A1
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P38606
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Expression Region
1-617aa
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AA Sequence
MDFSKLPKILDEDKESTFGYVHGVSGPVVTACDMAGAAMYELVRVGHSELVGEIIRLEGDMATIQVYEETSGVSVGDPVLRTGKPLSVELGPGIMGAIFDGIQRPLSDISSQTQSIYIPRGVNVSALSRDIKWDFTPCKNLRVGSHITGGDIYGIVSENSLIKHKIMLPPRNRGTVTYIAPPGNYDTSDVVLELEFEGVKEKFTMVQVWPVRQVRPVTEKLPANHPLLTGQRVLDALFPCVQGGTTAIPGAFGCGKTVISQSLSKYSNSDVIIYVGCGERGNEMSEVLRDFPELTMEVDGKVESIMKRTALVANTSNMPVAAREASIYTGITLSEYFRDMGYHVSMMADSTSRWAEALREISGRLAEMPADSGYPAYLGARLASFYERAGRVKCLGNPEREGSVSIVGAVSPPGGDFSDPVTSATLGIVQVFWGLDKKLAQRKHFPSVNWLISYSKYMRALDEYYDKHFTEFVPLRTKAKEILQEEEDLAEIVQLVGKASLAETDKITLEVAKLIKDDFLQQNGYTPYDRFCPFYKTVGMLSNMIAFYDMARRAVETTAQSDNKITWSIIREHMGDILYKLSSMKFKDPLKDGEAKIKSDYAQLLEDMQNAFRSLED
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Molecular Weight
93.5 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
ATP6V1A, a subunit of the vacuolar ATPase (V-ATPase) complex, plays a crucial role in cellular processes, including ion transport, pH regulation, and organelle acidification. This multi-subunit enzyme is essential for maintaining cellular homeostasis in various biological systems. Research has highlighted ATP6V1A's involvement in several physiological and pathological processes, such as bone resorption, cancer progression, and viral infections. Given its significant role in regulating lysosomal functions and autophagy, ATP6V1A has garnered attention as a potential therapeutic target for various diseases. The study of recombinant ATP6V1A protein enables scientists to elucidate its structural and functional properties, facilitating a deeper understanding of its mechanism of action. By producing and characterizing this protein, researchers aim to explore its interactions with other cellular components and its implications in disease contexts, paving the way for novel therapeutic strategies. Insights derived from ATP6V1A research may contribute to developing targeted treatments, enhancing the efficacy of existing therapies, and ultimately improving patient outcomes in conditions where V-ATPase activity is dysregulated. Thus, ATP6V1A stands as a vital focus in ongoing studies aimed at unraveling the complexities of cellular regulation and its impact on health and disease.
