Analytical Data
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基因名
PLEC1
- Application
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别名
EBS1; EBSO; HD1; Hemidesmosomal protein 1; PCN; pleC; PLEC_HUMAN; PLEC1; PLEC1b; Plectin 1; Plectin 1 intermediate filament binding protein 500kDa; Plectin 6; Plectin; Plectin-1; PLTN
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种属
Human
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表达系统
E. coli
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标签
GST-tag at N-terminal
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q15149
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表达区间
4384-4493 aa
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氨基酸序列
CGFEDPRTKTKMSAAQALKKGWLYYEAGQRFLEVQYLTGGLIEPDTPGRVPLDEALQRGTVDARTAQKLRDVGAYSKYLTCPKTKLKISYKDALDRSMVEEGTGLRLLEA
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分子量
37.84 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
PLEC1 (plectin-1) is a cytoskeletal protein that plays a critical role in maintaining cellular structure and integrity by serving as a versatile linker between various components of the cytoskeleton and membrane-bound proteins. Its significance is highlighted in various physiological processes, including cell adhesion, migration, and signaling. Recent studies have shown that alterations in PLEC1 expression can be associated with several pathological conditions, including cancer, where it may influence tumor progression and metastasis. Moreover, PLEC1 has been implicated in specific cellular responses to stress and inflammation, making it a potential candidate for therapeutic interventions. The recombinant production of PLEC1 facilitates a detailed investigation into its structural and functional properties, providing insights into its interaction with other intracellular partners and its mechanistic role in health and disease. This research is particularly relevant in the context of developing targeted therapies, where understanding the molecular basis of PLEC1's function could lead to novel approaches in treating diseases linked to cytoskeletal dysfunctions. Advances in protein engineering and purification techniques have enabled the generation of high-quality recombinant PLEC1, which is essential for biochemical assays and structural studies, paving the way for future investigations into its role in cellular dynamics and its potential as a biomarker for disease characterization.












