Analytical Data
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Gene name
Hadha
- Application
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Alternative Names
Hadha；HADH；Trifunctional enzyme subunit alpha. mitochondrial
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P40939
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Expression Region
37-763aa
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AA Sequence
TRTHINYGVKGDVAVVRINSPNSKVNTLSKELHSEFSEVMNEIWASDQIRSAVLISSKPGCFIAGADINMLAACKTLQEVTQLSQEAQRIVEKLEKSTKPIVAAINGSCLGGGLEVAISCQYRIATKDRKTVLGTPEVLLGALPGAGGTQRLPKMVGVPAALDMMLTGRSIRADRAKKMGLVDQLVEPLGPGLKPPEERTIEYLEEVAITFAKGLADKKISPKRDKGLVEKLTAYAMTIPFVRQQVYKKVEEKVRKQTKGLYPAPLKIIDVVKTGIEQGSDAGYLCESQKFGELVMTKESKALMGLYHGQVLCKKNKFGAPQKDVKHLAILGAGLMGAGIAQVSVDKGLKTILKDATLTALDRGQQQVFKGLNDKVKKKALTSFERDSIFSNLTGQLDYQGFEKADMVIEAVFEDLSLKHRVLKEVEAVIPDHCIFASNTSALPISEIAAVSKRPEKVIGMHYFSPVDKMQLLEIITTEKTSKDTSASAVAVGLKQGKVIIVVKDGPGFYTTRCLAPMMSEVIRILQEGVDPKKLDSLTTSFGFPVGAATLVDEVGVDVAKHVAEDLGKVFGERFGGGNPELLTQMVSKGFLGRKSGKGFYIYQEGVKRKDLNSDMDSILASLKLPPKSEVSSDEDIQFRLVTRFVNEAVMCLQEGILATPAEGDIGAVFGLGFPPCLGGPFRFVDLYGAQKIVDRLKKYEAAYGKQFTPCQLLADHANSPNKKFYQ
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Molecular Weight
86.0 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
Hadha, or 3-hydroxyacyl-CoA dehydrogenase, is a critical enzyme in the mitochondrial fatty acid oxidation pathway, playing a pivotal role in the metabolism of medium-chain and long-chain fatty acids. The study of Hadha focuses on its function in energy generation and metabolic regulation, particularly in the context of various metabolic disorders, including those associated with fatty acid oxidation defects. Mutations in the Hadha gene can lead to a range of health issues, including disorders like 3-hydroxyacyl-CoA dehydrogenase deficiency, which is characterized by impaired fatty acid metabolism and subsequent toxic accumulation of fatty acyl-CoA intermediates. The increasing prevalence of metabolic syndrome and related diseases has prompted researchers to investigate the structure, function, and regulation of Hadha, aiming to elucidate its role in normal physiology and disease. Moreover, understanding the mechanisms underlying Hadha's activity may contribute to the development of therapeutic strategies for metabolic diseases. Recent advances in proteomics and molecular biology techniques have allowed for in-depth studies of Hadha's enzymatic properties, potential regulatory mechanisms, and interactions with other metabolic pathways. This ongoing research is vital for identifying biomarkers and potential targets for intervention, as well as for establishing a comprehensive understanding of lipid metabolism and its implications for human health.
