Analytical Data
-
基因名
POLK
- Application
-
别名
DinB homolog 1 (E. coli); DINB protein; DINB1; DINP; DNA damage inducible protein b; DNA polymerase kappa; Polk; POLK_HUMAN; POLQ; Polymerase (DNA directed) kappa; polymerase. DNA. kappa
-
种属
Human
-
表达系统
E. coli
-
标签
GST-tag at N-terminal
-
纯度
Greater than 90% as determined by SDS-PAGE.
-
蛋白编号
Q9UBT6
-
表达区间
1-472 aa
-
氨基酸序列
MDSTKEKCDSYKDDLLLRMGLNDNKAGMEGLDKEKINKIIMEATKGSRFYGNELKKEKQVNQRIENMMQQKAQITSQQLRKAQLQVDRFAMELEQSRNLSNTIVHIDMDAFYAAVEMRDNPELKDKPIAVGSMSMLSTSNYHARRFGVRAAMPGFIAKRLCPQLIIVPPNFDKYRAVSKEVKEILADYDPNFMAMSLDEAYLNITKHLEERQNWPEDKRRYFIKMGSSVENDNPGKEVNKLSEHERSISPLLFEESPSDVQPPGDPFQVNFEEQNNPQILQNSVVFGTSAQEVVKEIRFRIEQKTTLTASAGIAPNTMLAKVCSDKNKPNGQYQILPNRQAVMDFIKDLPIRKVSGIGKVTEKMLKALGIITCTELYQQRALLSLLFSETSWHYFLHISLGLGSTHLTRDGERKSMSVERTFSEINKAEEQYSLCQELCSELAQDLQKERLKVLYFDMVSLVFKFFNSKMLP
-
分子量
77.66 kDa
-
内毒素
< 1.0 EU per μg protein as determined by the LAL method.
-
性状
Freeze-dried powder
-
缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
-
稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
POLK (Polymerase Kappa) is a member of the Y-family DNA polymerases, which are crucial for DNA repair and replication under stress conditions, such as during the bypass of DNA lesions. Studies have shown that POLK has unique properties that enable it to accurately incorporate nucleotides opposite damaged bases, which is essential for maintaining genomic stability. Its roles in translesion synthesis (TLS) have garnered significant attention, as incorrect replication can lead to mutations and contribute to diseases, including cancer. Moreover, POLK's involvement in various cellular processes, including cell cycle regulation and apoptosis, highlights its importance beyond DNA repair. With advancements in structural biology and biochemistry, researchers aim to elucidate POLK’s mechanisms of action, regulation, and interaction with other proteins involved in DNA metabolism. Understanding POLK could lead to novel therapeutic approaches, particularly in cancers where TLS pathways are dysregulated. As a target for drug development, detailed knowledge of POLK’s structure and function will be vital in designing interventions that could enhance genomic fidelity and combat cancer progression. Thus, POLK is a significant focus in current molecular biology research, providing insights into the fundamental processes of DNA damage response and repair.
