Analytical Data
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基因名
POLR3G
- Application
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别名
DNA directed RNA polymerase III 32 kDa polypeptide; DNA-directed RNA polymerase III subunit G; DNA-directed RNA polymerase III subunit RPC7; Polr3g; Polymerase (RNA) III (DNA directed) polypeptide G (32kD); RNA polymerase III 32 kDa subunit; RNA polymerase III subunit C7; RPC32; RPC7; RPC7_HUMAN
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
O15318
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表达区间
1-223 aa
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氨基酸序列
MAGNKGRGRA AYTFNIEAVG FSKGEKLPDV VLKPPPLFPD TDYKPVPLKT GEGEEYMLAL KQELRETMKR MPYFIETPEE RQDIERYSKR YMKVYKEEWI PDWRRLPREM MPRNKCKKAG PKPKKAKDAG KGTPLTNTED VLKKMEELEK RGDGEKSDEE NEEKEGSKEK SKEGDDDDDD DAAEQEEYDE EEQEEENDYI NSYFEDGDDF GADSDDNMDE ATY
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分子量
25.9 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
POLR3G (RNA polymerase III subunit G) is a crucial component of RNA polymerase III, which is responsible for synthesizing small RNAs, such as tRNA and 5S rRNA, that play essential roles in various cellular processes. Recent studies have highlighted its significance not only in transcription but also in regulating gene expression and maintaining genomic stability. Aberrations in POLR3G expression have been associated with various diseases, including cancer and neurodegenerative disorders. The investigation of POLR3G recombinant proteins has gained momentum as researchers aim to elucidate the molecular mechanisms underlying its function. By producing POLR3G in a recombinant system, scientists can analyze its structure, interaction with other protein components, and its role in the transcriptional machinery. Understanding these aspects could provide insights into the pathophysiology of diseases linked to POLR3G dysfunction and open new avenues for therapeutic intervention. Additionally, recombinant POLR3G can serve as a valuable tool in biotechnological applications, such as synthetic biology and RNA-based therapies, making its study a promising frontier in molecular biology and medicine.
