Analytical Data
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Gene name
POLM
- Application
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Alternative Names
POLM；polmu；DNA-directed DNA/RNA polymerase mu
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9NP87
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Expression Region
1-494aa
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AA Sequence
MLPKRRRARVGSPSGDAASSTPPSTRFPGVAIYLVEPRMGRSRRAFLTGLARSKGFRVLDACSSEATHVVMEETSAEEAVSWQERRMAAAPPGCTPPALLDISWLTESLGAGQPVPVECRHRLEVAGPRKGPLSPAWMPAYACQRPTPLTHHNTGLSEALEILAEAAGFEGSEGRLLTFCRAASVLKALPSPVTTLSQLQGLPHFGEHSSRVVQELLEHGVCEEVERVRRSERYQTMKLFTQIFGVGVKTADRWYREGLRTLDDLREQPQKLTQQQKAGLQHHQDLSTPVLRSDVDALQQVVEEAVGQALPGATVTLTGGFRRGKLQGHDVDFLITHPKEGQEAGLLPRVMCRLQDQGLILYHQHQHSCCESPTRLAQQSHMDAFERSFCIFRLPQPPGAAVGGSTRPCPSWKAVRVDLVVAPVSQFPFALLGWTGSKLFQRELRRFSRKEKGLWLNSHGLFDPEQKTFFQAASEEDIFRHLGLEYLPPEQRNA
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Molecular Weight
58.8 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
POLM, or DNA Polymerase Mu, is a member of the DNA polymerase family, which plays a vital role in DNA repair and synthesis processes within cells. Discovered in the late 1990s, POLM is particularly notable for its ability to perform non-homologous end joining (NHEJ), a critical pathway for repairing double-strand breaks in DNA, which can arise from various sources such as ionizing radiation, chemotherapy, or oxidative stress. Unlike other polymerases, POLM possesses unique features, including a terminal deoxynucleotidyl transferase activity, which allows it to add nucleotides to the ends of broken DNA, facilitating the repair process. This function is crucial for maintaining genomic stability and preventing mutations that could lead to cancer. Research has shown that POLM is involved in the repair of immunoglobulin gene segments during lymphocyte development and plays a role in V(D)J recombination, a process essential for generating diverse antibody repertoires. Furthermore, studies have indicated that dysregulation of POLM activity may contribute to tumorigenesis, making it a potential target for therapeutic intervention. Understanding the structural and functional properties of POLM, along with its involvement in DNA repair mechanisms, is critical for developing strategies to enhance genomic integrity and treat diseases related to DNA damage, including cancer. Given its importance in these cellular processes, POLM continues to be a subject of intensive research aimed at elucidating its molecular mechanisms and potential applications in clinical settings.
