Analytical Data
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Gene name
CHM
- Application
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Alternative Names
CHM；CHMI；LECT1；MYETS1；Leukocyte cell-derived chemotaxin 1
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P24386
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Expression Region
1-653aa
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AA Sequence
MADTLPSEFDVIVIGTGLPESIIAAACSRSGRRVLHVDSRSYYGGNWASFSFSGLLSWLKEYQENSDIVSDSPVWQDQILENEEAIALSRKDKTIQHVEVFCYASQDLHEDVEEAGALQKNHALVTSANSTEAADSAFLPTEDESLSTMSCEMLTEQTPSSDPENALEVNGAEVTGEKENHCDDKTCVPSTSAEDMSENVPIAEDTTEQPKKNRITYSQIIKEGRRFNIDLVSKLLYSRGLLIDLLIKSNVSRYAEFKNITRILAFREGRVEQVPCSRADVFNSKQLTMVEKRMLMKFLTFCMEYEKYPDEYKGYEEITFYEYLKTQKLTPNLQYIVMHSIAMTSETASSTIDGLKATKNFLHCLGRYGNTPFLFPLYGQGELPQCFCRMCAVFGGIYCLRHSVQCLVVDKESRKCKAIIDQFGQRIISEHFLVEDSYFPENMCSRVQYRQISRAVLITDRSVLKTDSDQQISILTVPAEEPGTFAVRVIELCSSTMTCMKGTYLVHLTCTSSKTAREDLESVVQKLFVPYTEMEIENEQVEKPRILWALYFNMRDSSDISRSCYNDLPSNVYVCSGPDCGLGNDNAVKQAETLFQEICPNEDFCPPPPNPEDIILDGDSLQPEASESSAIPEANSETFKESTNLGNLEESSE
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Molecular Weight
77.5 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
The research on CHM (Choroideremia) recombinant protein is primarily driven by the need to understand and develop therapies for choroideremia, a rare genetic disorder caused by mutations in the CHM gene, which is essential for proper functioning of the retinal cells. This progressive condition leads to vision loss due to the degeneration of the retinal pigment epithelium and photoreceptor cells. The study of CHM recombinant protein focuses on the production and characterization of the protein encoded by the CHM gene, allowing researchers to explore its biological functions and interactions within cellular pathways. Advances in recombinant DNA technology have facilitated the expression of this protein in model systems, offering insights into its role in cellular processes such as photoreceptor maintenance and survival. Furthermore, understanding the structure and function of CHM recombinant protein is crucial for the development of potential gene therapies, including gene replacement strategies that aim to restore normal function in affected individuals. These research efforts are complemented by the use of animal models and patient-derived cells, which provide an avenue for investigating the effects of CHM mutations and the efficacy of emerging therapeutic approaches. Overall, the study of CHM recombinant protein not only enhances our understanding of choroideremia but also contributes to the broader field of retinal disorders, paving the way for innovative treatments to combat vision loss.
