Analytical Data
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基因名
EN1
- Application
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别名
EN1；Homeobox Protein engrailed-1
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q05925
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表达区间
1-392aa
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氨基酸序列
MEEQQPEPKS QRDSALGAAA AATPGGLSLS LSPGASGSSG SGSDGDSVPV SPQPAPPSPP AAPCLPPLAH HPHLPPHPPP PPPQHLAAPA HQPQPAAQLH RTTNFFIDNI LRPDFGCKKE QPPPQLLVAA AARGGAGGGG RVERDRGQTA AGRDPVHPLG TRAPGAASLL CAPDANCGPP DGSQPAAAGA GASKAGNPAA AAAAAAAAVA AAAAAAAAKP SDTGGGGSGG GAGSPGAQGT KYPEHGNPAI LLMGSANGGP VVKTDSQQPL VWPAWVYCTR YSDRPSSGPR TRKLKKKKNE KEDKRPRTAF TAEQLQRLKA EFQANRYITE QRRQTLAQEL SLNESQIKIW FQNKRAKIKK ATGIKNGLAL HLMAQGLYNH STTTVQDKDE SE
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分子量
40.1 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
EN1 is a member of the engrailed family of transcription factors, known to play crucial roles in various biological processes, including neural development and the establishment of left-right asymmetry in vertebrates. Research into EN1 has gained significance due to its involvement in critical signaling pathways and potential implications in neurodevelopmental disorders and cancers. Studies have shown that EN1 expression is tightly regulated during developmental stages, and dysregulation of its function can lead to abnormal neuronal differentiation and other pathological conditions. Furthermore, EN1 interacts with several downstream targets, influencing gene expression patterns necessary for proper embryonic patterning and neural progenitor maintenance. The recombinant EN1 protein holds promise for investigating its functional roles in cellular models and understanding its mechanism of action. By producing EN1 in a recombinant system, researchers aim to facilitate the study of its properties, interactions, and potential therapeutic applications. The ongoing exploration of the structure-function relationships of EN1 will provide insights into its contributions to cellular processes and pathology, paving the way for targeted interventions in diseases where EN1 is implicated. This background sets the stage for further investigations aimed at delineating the multifaceted roles of EN1 in development and disease.
