Analytical Data
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Gene name
SMARCA2
- Application
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Alternative Names
SMARCA2；BAF190B；BRM；SNF2A；Probable global transcription activator SNF2L2
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P51531
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Expression Region
700-1216aa
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AA Sequence
SYYTVAHAISERVEKQSALLINGTLKHYQLQGLEWMVSLYNNNLNGILAD EMGLGKTIQTIALITYLMEHKRLNGPYLIIVPLSTLSNWTYEFDKWAPSV VKISYKGTPAMRRSLVPQLRSGKFNVLLTTYEYIIKDKHILAKIRWKYMI VDEGHRMKNHHCKLTQVLNTHYVAPRRILLTGTPLQNKLPELWALLNFLL PTIFKSCSTFEQWFNAPFAMTGERVDLNEEETILIIRRLHKVLRPFLLRR LKKEVESQLPEKVEYVIKCDMSALQKILYRHMQAKGILLTDGSEKDKKGK GGAKTLMNTIMQLRKICNHPYMFQHIEESFAEHLGYSNGVINGAELYRAS GKFELLDRILPKLRATNHRVLLFCQMTSLMTIMEDYFAFRNFLYLRLDGT TKSEDRAALLKKFNEPGSQYFIFLLSTRAGGLGLNLQAADTVVIFDSDWN PHQDLQAQDRAHRIGQQNEVRVLRLCTVNSVEEKILAAAKYKLNVDQKVI QAGMFDQKSSSHERRAF
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Molecular Weight
76 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
SMARCA2 (SWI/SNF-related, matrix-associated, actin-dependent regulator of chromatin, subfamily A, member 2) is a vital ATPase in the SWI/SNF chromatin remodeling complex, which plays a crucial role in regulating gene expression and maintaining chromatin structure. Alterations in SMARCA2 function have been implicated in various cancers and developmental disorders, highlighting its importance in cellular processes. The study of SMARCA2 recombinant proteins has gained attention due to their potential to elucidate the molecular mechanisms behind chromatin dynamics and tumorigenesis. Researchers are particularly interested in understanding how mutations or loss of SMARCA2 affects the chromatin landscape and gene regulatory networks. By expressing and purifying SMARCA2 as a recombinant protein, scientists can investigate its biochemical properties, interaction with other chromatin remodelers, and impact on transcriptional regulation. This research not only contributes to our understanding of basic cellular mechanisms but also provides insights into targeted therapeutic strategies for diseases associated with SMARCA2 dysregulation. The study of SMARCA2 can bridge gaps in our knowledge of chromatin biology and pave the way for novel interventions in cancer treatment and regenerative medicine.
