Analytical Data
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Gene name
RAD9A
- Application
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Alternative Names
Cell cycle checkpoint control protein; Cell cycle checkpoint control protein RAD9A; DNA repair exonuclease rad9 homolog A; hRAD 9; hRAD9; Rad 9; RAD 9A; RAD9 (S pombe) homolog ; RAD9 homolog A; RAD9 homolog; Rad9a; RAD9A_HUMAN
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q99638
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Expression Region
1-391 aa
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AA Sequence
MKCLVTGGNV KVLGKAVHSL SRIGDELYLE PLEDGLSLRT VNSSRSAYAC FLFAPLFFQQ YQAATPGQDL LRCKILMKSF LSVFRSLAML EKTVEKCCIS LNGRSSRLVV QLHCKFGVRK THNLSFQDCE SLQAVFDPAS CPHMLRAPAR VLGEAVLPFS PALAEVTLGI GRGRRVILRS YHEEEADSTA KAMVTEMCLG EEDFQQLQAQ EGVAITFCLK EFRGLLSFAE SANLNLSIHF DAPGRPAIFT IKDSLLDGHF VLATLSDTDS HSQDLGSPER HQPVPQLQAH STPHPDDFAN DDIDSYMIAM ETTIGNEGSR VLPSISLSPG PQPPKSPGPH SEEEDEAEPS TVPGTPPPKK FRSLFFGSIL APVRSPQGPS PVLAEDSEGE G
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Molecular Weight
42.5 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
RAD9A is an essential protein involved in the DNA damage response and repair mechanisms, playing a critical role in maintaining genomic stability. This protein is part of the 9-1-1 complex, which is activated in response to DNA damage and is crucial for cell cycle checkpoint activation and the facilitation of repair processes. Given its involvement in tumorigenesis, RAD9A has gained attention as a potential biomarker for cancer progression and a target for therapeutic intervention. Studies have indicated that overexpression of RAD9A is linked to various cancers, suggesting its role as a promoter of cancer cell proliferation and survival. Additionally, RAD9A interacts with several key regulatory proteins, influencing pathways related to apoptosis and DNA repair, making it a pivotal player in cellular responses to genotoxic stress. Hence, the study of RAD9A and its recombinant forms is crucial for understanding its precise biological functions, interactions, and potential applications in cancer therapy. Researchers aim to elucidate the molecular mechanisms underlying RAD9A's involvement in the DNA damage response, which may lead to novel strategies in cancer treatment and prevention. By generating recombinant RAD9A proteins, scientists can investigate their biochemical properties and interactions, aiding in the development of targeted therapies that exploit the vulnerabilities associated with its dysregulation in cancer cells.
