Analytical Data
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基因名
IpaD
- Application
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别名
IpaD；Invasin IpaD
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种属
E.coli
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P18013
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表达区间
1-332aa
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氨基酸序列
MNITTLTNSISTSSFSPNNTNGSSTETVNSDIKTTTSSHPVSSLTMLNDT LHNIRTTNQALKKELSQKTLTKTSLEEIALHSSQISMDVNKSAQLLDILS RNEYPINKDARELLHSAPKEAELDGDQMISHRELWAKIANSINDINEQYL KVYEHAVSSYTQMYQDFSAVLSSLAGWISPGGNDGNSVKLQVNSLKKALE ELKEKYKDKPLYPANNTVSQEQANKWLTELGGTIGKVSQKNGGYVVSINM TPIDNMLKSLDNLGGNGEVVLDNAKYQAWNAGFSAEDETMKNNLQTLVQK YSNANSIFDNLVKVLSSTISSCTDTDKLFLHF
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分子量
57 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
IpaD (Invasion Plasmid Antigen D) is a critical virulence factor secreted by Shigella flexneri, a pathogenic bacterium responsible for shigellosis, a severe form of bacterial dysentery. IpaD plays a vital role in the invasion process of epithelial cells by facilitating the formation of membrane protrusions that allow bacterial entry. Researchers have focused on characterizing IpaD due to its potential as a target for vaccine development and therapeutic interventions against Shigella infections. The protein is involved in the modulation of host cell signaling, which aids in bacterial entry and survival within the host. Additionally, IpaD interacts with various host proteins, leading to the activation of inflammatory pathways, further exacerbating tissue damage. Recent studies have employed recombinant DNA technology to produce IpaD for structural and functional analyses, enabling the elucidation of its mechanism of action and interactions with host cells. Understanding IpaD's role in Shigella pathogenesis is crucial for developing strategies to combat infections, as the bacterium exhibits increasing resistance to conventional antibiotics. Thus, the research into IpaD recombinant proteins not only contributes to fundamental microbiology but also holds promise for innovative public health solutions against bacterial diseases like shigellosis.
