Recombinant Human HGF Protein(HEK293), C-His

HGF

HGF Protein is a multifunctional glycoprotein. By binding to the transmembrane tyrosine kinase receptor Met, HGF Protein plays a key role in cell proliferation, migration, differentiation and tissue repair. Abnormalities of HGF Protein are closely associated with tumor growth, metastasis and organ fibrosis. HGF Protein, Human (HEK293, His) is a recombinant HGF protein expressed by HEK293 and tagged with C-His[1][2][3].

SPRMSTBLIITCELISACELL ASSAYDRUG SCREENING

1.The ability to induce IL-11 secretion by Saos-2 human osteosarcoma cells has an ED50 value of <2 ng/mL. 2.Immobilized Human HGF, His Tag at 1 μg/mL (100 μl/Well) on the plate. Dose response curve for Human HGF R, hFc Tag with the EC50 of ≤28.2 ng/mL determined by ELISA. 3. Measured by its binding ability in a functional ELISA. Immobilized Human HGF at 2 μg/mL (100 μL/well) can bind Human HGFR. The ED50 for this effect is <20 ng/mL. Measured by its ability to induce IL-11 secretion by Saos-2 human osteosarcoma cells. The ED50 for this effect is 1.6 ng/ml, corresponding to a specific activity is 6.25×105 U/mg. Measured by its binding ability in a functional ELISA. Immobilized Human HGF at 2 μg/mL (100 μL/well) can bind Human HGFR. The ED50 for this effect is 19.58 ng/mL.

Hepatocyte growth factor; HPTA; HGF; SF; Scatter factor; Hepatopoietin-A

Human

HEK293

C-His

Greater than 95% as determined by reducing SDS-PAGE.

P14210-1

Q32-S728

QRKRRNTIHEFKKSAKTTLIKIDPALKIKTKKVNTADQCANRCTRNKGLPFTCKAFVFDKARKQCLWFPFNSMSSGVKKEFGHEFDLYENKDYIRNCIIGKGRSYKGTVSITKSGIKCQPWSSMIPHEHSFLPSSYRGKDLQENYCRNPRGEEGGPWCFTSNPEVRYEVCDIPQCSEVECMTCNGESYRGLMDHTESGKICQRWDHQTPHRHKFLPERYPDKGFDDNYCRNPDGQPRPWCYTLDPHTRWEYCAIKTCADNTMNDTDVPLETTECIQGQGEGYRGTVNTIWNGIPCQRWDSQYPHEHDMTPENFKCKDLRENYCRNPDGSESPWCFTTDPNIRVGYCSQIPNCDMSHGQDCYRGNGKNYMGNLSQTRSGLTCSMWDKNMEDLHRHIFWEPDASKLNENYCRNPDDDAHGPWCYTGNPLIPWDYCPISRCEGDTTPTIVNLDHPVISCAKTKQLRVVNGIPTRTNIGWMVSLRYRNKHICGGSLIKESWVLTARQCFPSRDLKDYEAWLGIHDVHGRGDEKCKQVLNVSQLVYGPEGSDLVLMKLARPAVLDDFVSTIDLPNYGCTIPEKTSCSVYGWGYTGLINYDGLLRVAHLYIMGNEKCSQHHRGKVTLNESEICAGAEKIGSGPCEGDYGGPLVCEQHKMRMVLGVIVPGRGCAIPNRPGIFVRVAYYAKWIHKIILTYKVPQS

Full Length of Isoform-1 Mature Protein

30-40 kDa (β subunit) & 50-65 kDa (α subunit) & 80-100 kDa (α subunit+β subunit),based on SDS-PAGE under reducing conditions,.

< 1.0 EU per μg protein as determined by the LAL method.

Freeze-dried powder

PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

Site-directed mutagenesis Custom tag design Custom buffer formulation Custom full-length protein production

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.