Analytical Data
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Gene name
NQO1
- Application
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Alternative Names
NQO1；DIA4；NMOR1；NAD(P)H dehydrogenase [quinone] 1
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P15559
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Expression Region
2-274aa
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AA Sequence
VGRRALIVL AHSERTSFNY AMKEAAAAAL KKKGWEVVES DLYAMNFNPI ISRKDITGKL KDPANFQYPA ESVLAYKEGH LSPDIVAEQK KLEAADLVIF QFPLQWFGVP AILKGWFERV FIGEFAYTYA AMYDKGPFRS KKAVLSITTG GSGSMYSLQG IHGDMNVILW PIQSGILHFC GFQVLEPQLT YSIGHTPADA RIQILEGWKK RLENIWDETP LYFAPSSLFD LNFQAGFLMK KEVQDEEKNK KFGLSVGHHL GKSIPTDNQI KARK
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
NQO1 (NAD(P)H:quinone oxidoreductase 1) is an essential enzyme involved in the cellular defense mechanism against oxidative stress, playing a crucial role in the detoxification of electrophilic compounds and the protection of cells from damage caused by reactive oxygen species. Research has shown that NQO1 is crucial for maintaining cellular redox balance and has implications in various diseases, including cancer, neurodegenerative disorders, and cardiovascular diseases. Variants of the NQO1 gene have been linked to altered enzyme activity, influencing individual susceptibility to toxicants and the efficacy of chemotherapies. As a result, recombinant NQO1 protein has gained attention for its potential applications in drug development, biomarker discovery, and therapeutic interventions. The study of NQO1 involves various techniques, including gene cloning, protein expression systems, and purification methods, aiming to elucidate its structure-function relationship and regulatory mechanisms. Understanding the role of NQO1 in health and disease not only enhances our comprehension of oxidative stress management but also opens avenues for developing targeted therapies that could improve patient outcomes in conditions where NQO1 activity is compromised.
