Analytical Data
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Gene name
DAPK1
- Application
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Alternative Names
DAPK1；DAPK；Death-associated Protein kinase 1
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P53355
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Expression Region
2-363aa
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AA Sequence
TVFRQENVDDYYDTGEELGSGQFAVVKKCREKSTGLQYAAKFIKKRRTKS SRRGVSREDIEREVSILKEIQHPNVITLHEVYENKTDVILILELVAGGEL FDFLAEKESLTEEEATEFLKQILNGVYYLHSLQIAHFDLKPENIMLLDRN VPKPRIKIIDFGLAHKIDFGNEFKNIFGTPEFVAPEIVNYEPLGLEADMW SIGVITYILLSGASPFLGDTKQETLANVSAVNYEFEDEYFSNTSALAKDF IRRLLVKDPKKRMTIQDSLQHPWIKPKDTQQALSRKASAVNMEKFKKFAA RKKWKQSVRLISLCQRLSRSFLSRSNMSVARSDDTLDEEDSFVMKAIIHA INDDNVPGLQHL
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Molecular Weight
67 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
DAPK1 (Death-Associated Protein Kinase 1) is a serine/threonine kinase that plays a crucial role in various cellular processes, including apoptosis, autophagy, and cellular response to stress. It is particularly significant in the regulation of tumor suppression and has been implicated in the pathogenesis of various cancers. Research has shown that DAPK1 is often downregulated or inactivated in malignant tumors, contributing to tumor progression and resistance to therapy. The study of DAPK1 recombinant proteins is essential for understanding its functional mechanisms and interactions with other cellular pathways. By producing DAPK1 as a recombinant protein, researchers can investigate its enzymatic activity, identify potential substrates, and explore its role in signaling pathways associated with cancer. Additionally, recombinant DAPK1 can be utilized as a tool for drug discovery, enabling the screening of small molecules that may activate or inhibit its activity. The therapeutic potential of targeting DAPK1 in cancer treatment has garnered increasing interest, making it a significant focus for ongoing research. Insights obtained from DAPK1 recombinant protein studies may lead to the development of novel therapeutic strategies aimed at enhancing cancer cell sensitivity to treatment while further elucidating its role in normal cellular functions.
