Analytical Data
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Gene name
CAMK4
- Application
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Alternative Names
CAMK4；CAMK；CAMK-GR；CAMKIV；Calcium/calmodulin-dependent Protein kinase type IV
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q16566
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Expression Region
1-473aa
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AA Sequence
MGSSHHHHHHSSGLVPRGSHMGSHMLKVTVPSCSASSCSSVTASAAPGTA SLVPDYWIDGSNRDALSDFFEVESELGRGATSIVYRCKQKGTQKPYALKV LKKTVDKKIVRTEIGVLLRLSHPNIIKLKEIFETPTEISLVLELVTGGEL FDRIVEKGYYSERDAADAVKQILEAVAYLHENGIVHRDLKPENLLYATPA PDAPLKIADFGLSKIVEHQVLMKTVCGTPGYCAPEILRGCAYGPEVDMWS VGIITYILLCGFEPFYDERGDQFMFRRILNCEYYFISPWWDEVSLNAKDL VRKLIVLDPKKRLTTFQALQHPWVTGKAANFVHMDTAQKKLQEFNARRKL KAAVKAVVASSRLGSASSSHGSIQESHKASRDPSPIQDGNEDMKAIPEGE KIQGDGAQAAVKGAQAELMKVQALEKVKGADINAEEAPKMVPKAVEDGIK VADLELEEGLAEEKLKTVEEAAAPREGQGSSAVGFEVPQQDVILPEY
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Molecular Weight
55 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
CAMK4 (Calcium/Calmodulin-dependent Protein Kinase IV) is a serine/threonine kinase that plays a crucial role in various cellular processes, including gene expression, neuronal signaling, and immune responses. Its activation is primarily dependent on calcium influx and subsequent binding of calmodulin, which allows CAMK4 to translocate to the nucleus and phosphorylate various substrates, including transcription factors such as CREB (cAMP response element-binding protein). Dysregulation of CAMK4 has been implicated in a range of pathological conditions, including neurological disorders, cardiovascular diseases, and cancer. Research into the recombinant expression of CAMK4 is vital for elucidating its functional mechanisms, as well as for the development of potential therapeutic strategies targeting its pathways. Generating recombinant CAMK4 not only facilitates structural and functional studies but also allows for the examination of its interactions with other proteins and the downstream effects of its activity. Advances in recombinant technology have made it possible to produce CAMK4 in heterologous systems, leading to greater yields and more straightforward purification processes. These studies contribute to a deeper understanding of CAMK4's role in health and disease, and highlight its potential as a biomarker or therapeutic target. Overall, the investigation of recombinant CAMK4 is significant for both basic research and clinical applications, offering insights that could pave the way for innovative treatments for diverse ailments associated with calcium signaling and kinase activity.
